Effect of high glucose concentration on collagen synthesis and cholesterol level in the phenotypic modulation of aortic cultured smooth muscle cells of sand rat (Psammomys obesus).

S Aouichat Bouguerra, Y Benazzoug, F Bekkhoucha, M C Bourdillon
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引用次数: 12

Abstract

To simulate diabetic conditions, the effects of high glucose concentration on collagen synthesis and cholesterol level in cultured aortic smooth muscle cells of Psammomys were investigated. For collagen biosynthesis, smooth muscle cells (SMCs) were incubated in synthetic proliferative phase and in postconfluent phase with 3H-proline. Cellular cholesterol was determined by enzymatic method. Under high glucose concentration, the results showed morphological modifications characterized by morphometric cellular, nuclear, and nucleolar changes. In biochemical studies, the authors observed an increase of free and esterified cellular cholesterol as well as of total proteins, collagen biosynthesis, and alpha1 (I+III) and alpha2 (I) chains of collagen contained in the SMCs and in the extracellular matrix. These results showed the sensitivity of Psammomys aortic SMCs to high glucose concentration and would constitute an interesting cellular model to study atherosclerosis pathogeny in experimental diabetes.

高糖浓度对沙鼠主动脉培养平滑肌细胞表型调节中胶原合成和胆固醇水平的影响。
为了模拟糖尿病,研究了高葡萄糖浓度对培养的沙棘主动脉平滑肌细胞胶原合成和胆固醇水平的影响。对于胶原的生物合成,平滑肌细胞(SMCs)在合成增殖期和3h -脯氨酸的融合后期孵育。细胞胆固醇用酶法测定。在高葡萄糖浓度下,结果显示细胞、细胞核和核仁的形态改变。在生化研究中,作者观察到游离和酯化细胞胆固醇以及总蛋白、胶原生物合成和胶原蛋白α 1 (I+III)和α 2 (I)链在SMCs和细胞外基质中的增加。这些结果显示了Psammomys主动脉SMCs对高葡萄糖浓度的敏感性,为研究实验性糖尿病动脉粥样硬化的发病机制提供了一个有趣的细胞模型。
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