Properties of the hydrogenase of scenedesmus

Abstract

• 1.

  1. The adaptation of hydrogenase (EC 1.98.1.1) in Scenedesnus obliquus was inhibited by dithionite, sulfite, arsenite, marphaside, BAL, 2,2′-dipyridyl, and 1,10-phenanthroline. None of these inhibitors had any effect on the active hydrogenase after adaptation.

• 2.

  2. The active hydrogenase of Scenedesmus was inhibited by silver, mercury, and cupric ions, p-chloromercuribenzoate and iodoacetamide. The silver and mercury inhibition could be reversed by BAL.

• 3.

  3. The hydrogenase of Proteus vulgaris was not affected by the inhibitors of the adaptation process but was inhibited by those reagents which inhibited the active hydrogenase of Scenedesmus.