Resolution of rabbit γ-globulin into two fractions by chromatography on diethylaminoethyl-sephadex

Michael Sela, David Givol, Edna Mozes
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引用次数: 37

Abstract

Rabbit γ-globulin was resolved into two fractions by chromatography of normal or immune serum on a DEAE-Sephadex column at pH 8. Different distribution of antibodies from the different antisera among the two γ-globulin fractions was observed.

Upon hydrolysis by papain followed by separation of the fragments formed, both γ-globulin fractions yielded the same amount of fragment III; one of them gave in addition a double amount of fragment II and the other a double amount of fragment I. This provides additional evidence that γ-globulin molecules are composed of two identical pieces (fragments I or II) together with fragment III.

二乙胺乙基葡聚糖层析法分离兔γ-球蛋白
用DEAE-Sephadex色谱柱在pH 8下将正常血清和免疫血清分离成兔γ-球蛋白两部分。不同抗血清的抗体在两组γ-球蛋白中分布不同。经木瓜蛋白酶水解并分离所形成的片段,两个γ-球蛋白组分产生相同数量的片段III;其中一种还提供了两倍量的片段II,另一种提供了两倍量的片段I。这进一步证明了γ-球蛋白分子是由两个相同的片段(片段I或片段II)和片段III组成的。
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