The distribution of muscle antigens in contracted myofibrils determined by fluorescein-labeled antibodies.

B TUNIK, H HOLTZER
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引用次数: 31

Abstract

Chick myofibrils in different states of contraction were treated with fluorescein-labeled antibodies. The rabbit antibodies were prepared against chick myosin, light and heavy meromyosins, and actin. For any one state of contraction, a single myofibril was photographed through the phase contrast microscope, stained with one of the antisera, and photographed through the fluorescence microscope. The cytological changes in the sarcomeres accompanying contraction as observed under phase were correlated with changes in the distribution of the precipitated antibodies as observed under the fluorescence microscope. The changing patterns observed through the fluorescence microscope were compared with those predicted by the sliding filament model of contraction.

用荧光素标记抗体测定收缩肌原纤维中肌肉抗原的分布。
用荧光素标记抗体处理不同收缩状态的鸡肌原纤维。制备了鸡肌球蛋白、轻、重肌球蛋白和肌动蛋白的兔抗体。对于任何一种收缩状态,通过相差显微镜拍摄单个肌原纤维,用一种抗血清染色,并通过荧光显微镜拍摄。相下观察到的肌瘤随收缩的细胞学变化与荧光显微镜下观察到的沉淀抗体分布变化相关。通过荧光显微镜观察到的变化模式与滑动丝收缩模型预测的变化模式进行了比较。
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