Ultraviolet Photometric Assay of Thiopental and Pentobarbital in Blood and Plasma*

Abstract

A procedure for the determination of sodium pentobarbital and sodium thiopental under rigidly standardized conditions of extraction, using Allen's absorption factor, is described. This method makes possible the reliable quantitative determination of 1 mcg./ml. or smaller concentration differences. In an appendix Allen's absorption factor for sodium pentobarbital is derived.