Serum fractionation and the effects of bovine serum fractions on human cells grown in a chemically defined medium.

The Journal of Biophysical and Biochemical Cytology Pub Date : 1961-07-01 DOI:10.1083/jcb.10.3.389

R HOLMES, S W WOLFE

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引用次数: 27

Abstract

Serum has been fractionated by curtain electrophoresis using carboxymethyl cellulose dissolved in sodium bicarbonate electrolyte. Various fractions were produced from bovine serum and added to replicate cultures of Chang's endoepithelial cells and HeLa cells grown in a chemically defined medium. The effects of each of the various fractions on the appearance of the cultures and on cell multiplication were studied. Three different fractions were obtained and two were subjected to further purification. One fraction associated with albumin promoted survival, attachment, and flattening as well as cell multiplication. A second fraction associated with the alpha globulins promoted survival and multiplication of some cells. A third fraction caused cells to aggregate and form free floating clumps. An adequate chemically defined medium for continuous growth of human cells was used throughout the study. The response of cells to alterations in their environment which simulated some of the effects produced by serum fractions is described.

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血清分离和牛血清对在化学确定的培养基中生长的人细胞的影响。

用溶解在碳酸氢钠电解质中的羧甲基纤维素幕电泳分离血清。从牛血清中提取不同的组分，并将其添加到在化学定义的培养基中生长的张氏内皮细胞和HeLa细胞的重复培养中。研究了不同组分对培养物外观和细胞增殖的影响。得到三种不同的馏分，其中两种进行进一步纯化。一个与白蛋白相关的部分促进了存活、附着、扁平以及细胞增殖。与α球蛋白相关的第二部分促进了某些细胞的存活和增殖。第三个部分导致细胞聚集并形成自由漂浮的团块。在整个研究过程中，使用了一种适当的化学定义的培养基，用于人类细胞的连续生长。描述了细胞对环境变化的反应，模拟了血清组分产生的一些影响。

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The Journal of Biophysical and Biochemical Cytology

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