N Doménech, B Alvarez, R Bullido, F Alonso, A Ezquerra, J Domínguez
{"title":"A new epitope on swine CD5 molecule detected by monoclonal antibody 5F12/9.","authors":"N Doménech, B Alvarez, R Bullido, F Alonso, A Ezquerra, J Domínguez","doi":"10.1089/153685903322286601","DOIUrl":null,"url":null,"abstract":"<p><p>This paper describes the production and characterization of a monoclonal antibody (MAb), 5F12/9, that recognizes a new epitope on porcine CD5. Conformation of its CD5 specificity was obtained by means of sequential immunoprecipitation and Western blot experiments in combination with anti-CD5 MAb 1H6/8, whereas cross-blocking experiments with both MAbs showed that they reacted with different epitopes.</p>","PeriodicalId":83733,"journal":{"name":"Hybridoma and hybridomics","volume":"22 3","pages":"179-82"},"PeriodicalIF":0.0000,"publicationDate":"2003-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/153685903322286601","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Hybridoma and hybridomics","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1089/153685903322286601","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 1
Abstract
This paper describes the production and characterization of a monoclonal antibody (MAb), 5F12/9, that recognizes a new epitope on porcine CD5. Conformation of its CD5 specificity was obtained by means of sequential immunoprecipitation and Western blot experiments in combination with anti-CD5 MAb 1H6/8, whereas cross-blocking experiments with both MAbs showed that they reacted with different epitopes.