Incorporation of nonnatural amino acids into proteins by using five-base codon-anticodon pairs.

T Hohsaka, M Sisido
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引用次数: 4

Abstract

A novel strategy for the incorporation of nonnatural amino acids into proteins was developed by using five-base codon-anticodon pairs. The streptavidin mRNA containing five-base codon CGGUA and the chemically aminoacylated tRNA with five-base anticodon UACCG were prepared, and added into E. coli in vitro translation system. As a result, the nonnatural amino acid was successfully incorporated into desired position of the protein. Other five-base codons CGGN1N2, where N1 and N2 indicate one of four nucleotides, were also available for the incorporation of the nonnatural amino acid.

利用五碱基密码子-反密码子对将非天然氨基酸整合到蛋白质中。
通过使用五碱基密码子-反密码子对,开发了一种将非天然氨基酸整合到蛋白质中的新策略。制备了含有五碱基密码子CGGUA的链亲和素mRNA和含有五碱基反密码子UACCG的化学氨基化tRNA,并将其加入大肠杆菌体外翻译体系。结果,非天然氨基酸被成功地结合到蛋白质的所需位置。其他五碱基密码子CGGN1N2,其中N1和N2表示四种核苷酸中的一种,也可用于非天然氨基酸的结合。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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