Smart probe: a novel fluorescence quenching-based oligonucleotide probe carrying a fluorophore and an intercalator.

A Yamane
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引用次数: 5

Abstract

A novel probe (Smart probe) has been developed for nucleic acid detection. The smart probe is an oligodeoxyribonucleotide carrying a fluorophore and an intercalator internally. Fluorescence of the smart probe is quenched by the intercalator in the absence of target sequence. While upon hybridization the probe emits greater fluorescence due to the interference of quenching by intercalation. The smart probe has been shown to recognize a single base mismatch in the double-stranded form without utilizing thermal stability difference of hybrids.

智能探针:一种基于荧光猝灭的新型寡核苷酸探针,携带荧光团和插层器。
研制了一种新型核酸检测探针(智能探针)。该智能探针是一种寡脱氧核糖核苷酸,其内部携带一个荧光团和一个插层。在没有目标序列的情况下,智能探针的荧光被插层器猝灭。而在杂交时,由于插层猝灭的干扰,探针发出更大的荧光。智能探针已被证明可以识别双链形式的单碱基不匹配,而无需利用杂化物的热稳定性差异。
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