A Mahara, R Iwase, K Yamana, T Yamaoka, A Murakami
{"title":"Characterization of RNA structure by bis-pyrene-labeled 2'-O-methyloligonucleotides.","authors":"A Mahara, R Iwase, K Yamana, T Yamaoka, A Murakami","doi":"10.1093/nass/44.1.199","DOIUrl":null,"url":null,"abstract":"<p><p>Properties of 2'-O-methyloligoribonucleotides containing two consecutive 2'-O-(1-pyrenylmethyl)uridine were investigated as a fluorescent probe to search the single strand regions of RNA. The bis-pyrene-labeled 2'-O-methyloligoribonucleotide (OMUpy2) induced the formation of pyrene dimer upon hybridization with the complementary oligoribonucleotides and showed remarkable appearance of broad structureless fluorescence at 480 nm. Contrarily, when OMUpy2 was hybridized with the complementary oligodeoxyribonucleotides, such enhancement of fluorescence was scarcely observed. When various OMUpy2 were applied to E. coli 5S-rRNA, the fluorescence intensity at 480 nm was varied in a sequence specific manner.</p>","PeriodicalId":19394,"journal":{"name":"Nucleic acids symposium series","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1093/nass/44.1.199","citationCount":"2","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Nucleic acids symposium series","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1093/nass/44.1.199","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 2
Abstract
Properties of 2'-O-methyloligoribonucleotides containing two consecutive 2'-O-(1-pyrenylmethyl)uridine were investigated as a fluorescent probe to search the single strand regions of RNA. The bis-pyrene-labeled 2'-O-methyloligoribonucleotide (OMUpy2) induced the formation of pyrene dimer upon hybridization with the complementary oligoribonucleotides and showed remarkable appearance of broad structureless fluorescence at 480 nm. Contrarily, when OMUpy2 was hybridized with the complementary oligodeoxyribonucleotides, such enhancement of fluorescence was scarcely observed. When various OMUpy2 were applied to E. coli 5S-rRNA, the fluorescence intensity at 480 nm was varied in a sequence specific manner.
研究了含有两个连续2'- o -(1-芘基甲基)尿嘧啶的2'- o -甲基寡核苷酸的性质，作为荧光探针搜索RNA单链区域。双芘标记的2'- o -甲基寡核苷酸(OMUpy2)与互补的寡核苷酸杂交后诱导形成芘二聚体，并在480 nm处显示出明显的宽无结构荧光。相反，当OMUpy2与互补的寡脱氧核糖核苷酸杂交时，几乎没有观察到这种荧光增强。当不同的OMUpy2作用于大肠杆菌5S-rRNA时，在480 nm处的荧光强度以序列特异性的方式变化。
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
