Analysis of double-strand-break repair by Chlorella retrotransposon Zepp.

Y Yamamoto, Y Noutoshi, M Fujie, S Usami, T Yamada
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引用次数: 2

Abstract

To elucidate the contribution of LINE-like retrotransposon Zepps in formation and maintenance of chromosomal telomeres, newly formed mini-chromosomes in irradiated Chlorella vulgaris cells were isolated and structurally characterized. A mini-chromosome Y32 (approximately 400 kbp in size) was shown to have several copies of Zepp elements on both termini. On the right arm terminus, two copies of Zepps were found in a tandem array with poly(A) tracts facing towards the chromosome end. The poly(A) tail and a 3'-end of approximately 400 bp of the distal copy was replaced by telomeric repeats. On 5'-side of the proximal copy was another Zepp element in a reversed orientation. This newly formed telomeric structure is very similar to that found in the left arm terminus of chromosome I and support the model of Zepp-mediated maintenance of Chlorella telomeres.

小球藻反转录转座子Zepp修复双链断裂的分析。
为了阐明线样反转录转座子Zepps在染色体端粒形成和维持中的作用,我们对辐照小球藻细胞中新形成的微染色体进行了分离和结构鉴定。迷你染色体Y32(大小约为400 kbp)在两端都有几个Zepp元件的拷贝。在右臂末端,发现两个Zepps拷贝呈串联阵列,poly(a)束朝向染色体末端。远端拷贝的poly(A)尾部和约400bp的3'端被端粒重复序列所取代。在近端拷贝的5'侧有另一个方向相反的Zepp元件。这种新形成的端粒结构与I号染色体左臂末端的结构非常相似,支持zepp介导的小球藻端粒维持模型。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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