Single long DNA molecule analysis using fluorescence microscopy.

N Kaji, M Ueda, Y Baba
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引用次数: 1

Abstract

Single long DNA molecule (T4 DNA) in agarose gel was visualized with a fluorescence microscope. We confirmed alternating current electric fields is effective for stretching of single DNA molecule in agarose gel. This stretching phenomenon was observed with wide range of agarose gel concentration from 0.5%(W/V) to 1.5%. From this observation, the presence of agarose gel fiber is essential for this stretching phenomenon. The stretching process of several DNA molecules in gel shows discontinuity, which is never observed in polymer systems. It would be based on topological restriction from gel fibers.

单长DNA分子荧光显微镜分析。
用荧光显微镜观察琼脂糖凝胶中的单个长DNA分子(T4 DNA)。我们证实了交流电场对琼脂糖凝胶中单个DNA分子的拉伸是有效的。琼脂糖凝胶浓度在0.5%(W/V) ~ 1.5%范围内均可观察到这种拉伸现象。从这个观察,琼脂糖凝胶纤维的存在是必不可少的这种拉伸现象。几个DNA分子在凝胶中的拉伸过程显示出不连续性,这在聚合物系统中从未观察到。它将基于凝胶纤维的拓扑限制。
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