Improvement of IGCR technique using FRET.

T Yasukochi, A Ooyama, H Kumazawa, J Kawanabe, K Ozawa, Y Shibanaka
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Abstract

The fluorescent detection system has been introduced into the study on denaturation/reassociation process of DNA fragments in gel, for improving In-Gel Competitive Reassociation technique, one of genome subtraction methods. The annealing behaviour of the mixture of 3'-Fluorescein-labelled and 5'-Cy5-labelled DNA fragments was analysed by Fluorescence Resonance Energy Transfer (FRET) technique from donor Fluorescein to acceptor Cy5. We showed that two fluorescent dyes labelled at 3' and 5' ends of DNA fragments caused FRET in both the solution and the gel. The characterisation of fluorescence-labelled fragments in gel and the changes of their fluorescence intensity will be reported.

利用FRET技术改进IGCR技术。
将荧光检测系统引入到凝胶中DNA片段变性/再结合过程的研究中,以改进凝胶内竞争性再结合技术这一基因组减法方法之一。用荧光共振能量转移(FRET)技术分析了3'-荧光素标记的DNA片段和5'-Cy5标记的DNA片段混合物的退火行为。我们发现在DNA片段的3'和5'端标记的两种荧光染料在溶液和凝胶中都引起了FRET。本文将报道凝胶中荧光标记片段的特征及其荧光强度的变化。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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