[The primary culture methods of colorectal adenoma cells].

Abstract

Objective: To improve the primary cell culture methods of colorectal adenomas.

Methods: The methods of the in vitro primary cell culture of colorectal adenomas, mainly the method for taking and preparing specimen, the methods of culture conditions and cells purification, were considered again and improved in this study.

Results: Of the 32 cases, of polyp, 26 were successfully cultured and 6 failed. The culture lasted 5-23 days with 50%-95% viscosity of wells. By means of immuno-histochemical staining and electromicroscopy, the cultured cells were proved to be of epithelial origin.

Conclusion: The primary culture methods of adenoma cells have been improved, but further study on the serial transfer culture cell lines is most needed.