[Osteocalcin and type I collagen mRNA expression of rat marrow stromal cells under induction condition].

Hua xi yi ke da xue xue bao = Journal of West China University of Medical Sciences = Huaxi yike daxue xuebao Pub Date : 2002-04-01

Huaiqing Chen, Jun Han, Liang Li, Jianghua Lu, Wensheng Zhang, Rui Chen

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Abstract

Objective: To investigate osteocalcin and type I collagen mRNA expression of rat marrow stromal cells under induction condition.

Methods: Cultured rat marrow stromal cells were induced by osteogenic compound(composed of dexamethasone 10(-3) mol/L, beta glyceralphosphate 10 mmol/L, ascorbic acid 50 micrograms/ml). Osteocalcin and type I collagen mRNA expression were measured using RT-PCR technique.

Results: It was found that rat marrow stromal cells in induced group have an increased time-dependent expression of osteocalcin and type I collagen mRNA, in comparison with control groups. And the necessity of 1,25-(OH)2D3 in induced osteocalcin mRNA expression was confirmed.

Conclusion: The cultured rat marrow stromal cells kept the undifferentiated state and had osteogenic potential testified by increased osteocalcin and type I collagen mRNA expression when induced.

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[诱导条件下大鼠骨髓基质细胞骨钙素和I型胶原mRNA的表达]。

目的:观察诱导条件下大鼠骨髓基质细胞骨钙素和I型胶原mRNA的表达。方法:用成骨化合物(由地塞米松10(-3)mol/L、甘油磷酸酯10 mmol/L、抗坏血酸50微克/ml组成)诱导培养的大鼠骨髓基质细胞。采用RT-PCR技术检测骨钙素和I型胶原mRNA的表达。结果:与对照组相比，诱导组大鼠骨髓基质细胞骨钙素和I型胶原mRNA的时间依赖性表达增加。证实了1,25-(OH)2D3在诱导骨钙素mRNA表达中的必要性。结论:培养的大鼠骨髓基质细胞保持未分化状态，具有成骨潜能，诱导后骨钙素和I型胶原mRNA表达增加。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Hua xi yi ke da xue xue bao = Journal of West China University of Medical Sciences = Huaxi yike daxue xuebao

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