Effects of 630-, 660-, 810-, and 905-nm laser irradiation delivering radiant exposure of 1-50 J/cm2 on three species of bacteria in vitro.

Ethne L Nussbaum, Lothar Lilge, Tony Mazzulli
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引用次数: 139

Abstract

Objective: To examine the effects of low-intensity laser therapy (LILT) on bacterial growth in vitro.

Background data: LILT is undergoing investigation as a treatment for accelerating healing of open wounds. The potential of coincident effects on wound bacteria has received little attention. Increased bacterial proliferation could further delay recovery; conversely inhibition could be beneficial.

Materials and methods: Pseudomonas aeruginosa, Escherichia coli, and Staphylococcus aureus were plated on agar and then irradiated with wavelengths of 630, 660, 810, and 905 nm (0.015 W/cm(2)) and radiant exposures of 1-50 J/cm(2). In addition, E. coli was irradiated with 810 nm at an irradiance of 0.03 W/cm(2) (1-50 J/cm(2)). Cells were counted after 20 h of incubation post LILT. Repeated measures ANOVA and Tukey adjusted post hoc tests were used for analysis.

Results: There were interactions between wavelength and species (p = 0.0001) and between wavelength and radiant exposure (p = 0.007) in the overall effects on bacterial growth; therefore, individual wavelengths were analyzed. Over all types of bacteria, there were overall growth effects using 810- and 630-nm lasers, with species differences at 630 nm. Effects occurred at low radiant exposures (1-20 J/cm(2)). Overall effects were marginal using 660 nm and negative at 905 nm. Inhibition of P. aeruginosa followed irradiation using 810 nm at 5 J/cm(2) (-23%; p = 0.02). Irradiation using 630 nm at 1 J/cm(2) inhibited P. aeruginosa and E. coli (-27%). Irradiation using 810 nm (0.015 W/cm(2)) increased E. coli growth, but with increased irradiance (0.03 W/cm(2)) the growth was significant (p = 0.04), reaching 30% at 20 J/cm(2) (p = 0.01). S. aureus growth increased 27% following 905-nm irradiation at 50 J/cm(2).

Conclusion: LILT applied to wounds, delivering commonly used wavelengths and radiant exposures in the range of 1-20 J/cm(2), could produce changes in bacterial growth of considerable importance for wound healing. A wavelength of 630 nm appeared to be most commonly associated with bacterial inhibition. The findings of this study might be useful as a basis for selecting LILT for infected wounds.

630、660、810和905 nm激光照射1-50 J/cm2对三种体外细菌的影响
目的:探讨低强度激光治疗(LILT)对体外细菌生长的影响。背景资料:LILT作为一种加速开放性伤口愈合的治疗方法正在进行研究。对伤口细菌的潜在协同作用很少受到关注。细菌增殖增加可能进一步延迟恢复;相反,抑制可能是有益的。材料和方法:将铜绿假单胞菌、大肠杆菌和金黄色葡萄球菌分别镀于琼脂上,分别以波长630、660、810和905 nm (0.015 W/cm(2))和1-50 J/cm(2)的辐射照射。另外,对大肠杆菌进行810 nm辐照,辐照度为0.03 W/cm(2) (1 ~ 50 J/cm(2))。在LILT孵育20 h后计数细胞。采用重复测量方差分析和Tukey校正事后检验进行分析。结果:波长与菌种之间存在交互作用(p = 0.0001),波长与辐射暴露之间存在交互作用(p = 0.007);因此,对单个波长进行了分析。在所有类型的细菌中,使用810 nm和630 nm激光有总体生长效果,在630 nm有物种差异。在低辐射照射(1-20 J/cm(2))下发生影响。使用660 nm时,总体效果为边际效应,使用905 nm时为负效应。5 J/cm(2) 810 nm辐照后铜绿假单胞菌的抑制作用(-23%;P = 0.02)。以1j /cm(2)照射630 nm,可抑制铜绿假单胞菌和大肠杆菌(-27%)。810 nm (0.015 W/cm(2))辐照能促进大肠杆菌的生长,但随着辐照强度(0.03 W/cm(2))的增加,大肠杆菌的生长显著(p = 0.04),在20 J/cm(2)时达到30% (p = 0.01)。50j /cm照射905 nm后金黄色葡萄球菌生长增加27%(2)。结论:应用于伤口的LILT,提供1-20 J/cm(2)范围内的常用波长和辐射暴露,可以产生对伤口愈合相当重要的细菌生长的变化。630 nm的波长似乎最常与细菌抑制有关。本研究结果可作为选择LILT治疗感染创面的依据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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