An improved method for rapid ABO genotyping.

Sbornik lekarsky Pub Date : 2002-01-01

D Vanĕk, M Pospísek

引用次数: 0

Abstract

A method for ABO genotyping originally designed by Lee and Chang [3] and further developed by Akane et al. [7] has been even more simplified and improved. We obtained a rapid, robust, sensitive and low cost method for detection of sequence polymorphism of ABO glycosyltransferase gene by changing the MaeII restriction enzyme for its isoschizomer TaiI and by optimization of the condition during digestion and electrophoretic separation.

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一种改进的快速ABO基因分型方法。

由Lee和Chang[3]最初设计，Akane等人[7]进一步发展的ABO基因分型方法更加简化和完善。通过改变ABO糖基转移酶同分异构体TaiI的MaeII限制性内切酶，优化酶切和电泳分离条件，获得了一种快速、可靠、灵敏、低成本的ABO糖基转移酶基因序列多态性检测方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Sbornik lekarsky

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