Occurrence of the lung fluke Paragonimus hueit'ungensis in Manipur, India.

T Shantikumar Singh
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Abstract

Background: Paragonimus has emerged as an important parasitic zoonosis in recent years in Manipur, India. However, no scientific data on the systematic and phylogenetic classification of lung fluke in India is available. Therefore, a study was conducted to determine the species of lung fluke prevalent in Manipur by infecting an experimental animal with Paragonimus metacercariae obtained from fresh water crabs.

Methods: Thirteen Paragonimus metacercariae isolated from the fresh water crabs, Potamiscus manipurensis, at Churachandpur, Manipur, India were used to infect a puppy orally using a Pasteur pipette. After the infection, fecal specimens were checked for Paragonimus eggs by microscopic examination. Autopsy was performed on day 121 post-infection. The worms recovered from the worm cysts were alcohol fixed, flattened and stained with carmine for morphological study.

Results: Four adult worms were recovered. The worms presented with singly spaced cuticular spines, and the ventral suckers were slightly larger than the oral suckers. The ovaries consisted of 4 to 5 lobed masses branched like a ginger stem, and the testes were irregularly lobed and elongated. One ofthe worms showed a different shape and size ofthe right testis. The morphological features of the worms are similar to the Chinese species, Paragonimus hueit'ungensis.

Conclusion: The results of this study indicate the possibility of occurrence of P hueit'ungensis as 1 of the important Paragonimus species prevalent in Manipur, India after its initial discovery in China.

印度曼尼普尔邦肺吸虫的发生。
背景:吸虫是近年来在印度曼尼普尔邦出现的一种重要的寄生虫病。然而,在印度没有关于肺吸虫的系统和系统发育分类的科学数据。因此,本研究采用从淡水蟹中获得的肺吸虫感染实验动物,以确定曼尼普尔流行的肺吸虫种类。方法:采用巴斯德移液管对印度曼尼普尔邦Churachandpur的淡水蟹(Potamiscus manipurensis)中分离的13株吸虫进行口腔感染。感染后,用显微镜检查粪便标本中是否有肺吸虫卵。感染后第121天进行尸检。用酒精固定,压平,胭脂红染色进行形态学研究。结果:共捕获成虫4只。线虫呈单间距角质层棘,腹吸盘略大于口吸盘。子房由4 ~ 5个裂片组成,呈姜茎状分枝,睾丸不规则裂片,呈细长状。其中一只蠕虫显示出不同形状和大小的右睾丸。该蠕虫的形态特征与中国种hueit'ungensis相似。结论:P hueit'ungensis在中国首次发现后,作为印度曼尼普尔邦流行的重要吸虫种之一,可能在印度曼尼普尔邦存在。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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