Cytotoxic effects of gossypol and vitamin E on human and rat lymphocytes and spermatozoa.

Abstract

The cytotoxicity of vitamin E, gossypol, and mixture of both was determined in lymphocytes and spermatozoa using different concentrations of the substances. The cellular viability was assessed by means of the trypan blue exclusion test at 0, 60, and 120 minutes. Spermatozoa exhibited a higher sensitivity to these compounds, which was proportional to the length of exposure and the dose.