Effect of catecholamine depletion on oxidative energy metabolism in rat liver, brain and heart mitochondria; use of reserpine

Comparative Biochemistry and Physiology Part C: Pharmacology, Toxicology and Endocrinology Pub Date : 2000-08-01 DOI:10.1016/S0742-8413(00)00134-1

Vaishali H. Shukla , Kunjan R. Dave , Surendra S. Katyare

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引用次数: 22

Abstract

Regulation of mitochondrial functions in vivo by catecholamines was examined indirectly by depleting the catecholamines stores by reserpine treatments of the experimental animals. Reserpine treatment resulted in decreased respiratory activity in liver and brain mitochondria with the two NAD⁺-linked substrates: glutamate and pyruvate+malate with succinate ATP synthesis rate decreased in liver mitochondria only. With ascorbate+TMPD system, the ADP/O ratio and ADP phosphorylation rate decreased in brain mitochondria. For the heart mitochondria, state 3 respiration rates decreased for all substrates. In the liver mitochondria basal ATPase activity decreased by 51%, but in the presence of Mg²⁺ and/or DNP increased significantly. In the brain and heart mitochondria ATPase activities were unchanged. The energy of activation in high temperature range increased liver mitochondrial ATPase while in brain mitochondria reserpine treatment resulted in abolishment in phase transition. Total phospholipid (TPL) content of the brain mitochondria increased by 22%. For the heart mitochondria TPL content decreased by 19% and CHL content decreased by 34%. Tissue specific differential effects were observed for the mitochondrial phospholipid composition. Liver mitochondrial membranes were more fluidized in the reserpine-treated group. The epinephrine and norepinephrine contents in the adrenals decreased by 68 and 77% after reserpine treatment.

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儿茶酚胺耗竭对大鼠肝、脑、心线粒体氧化能代谢的影响利血平的使用

通过利血平处理消耗实验动物的儿茶酚胺储存，间接检查了儿茶酚胺在体内对线粒体功能的调节。利血平治疗导致肝脏和脑线粒体呼吸活性下降，两种NAD+连接底物:谷氨酸和丙酮酸+苹果酸，仅在肝脏线粒体中琥珀酸ATP合成率下降。在抗坏血酸+TMPD体系下，脑线粒体中ADP/O比值和ADP磷酸化率降低。对于心脏线粒体，所有底物的状态3呼吸速率都降低。肝脏线粒体基础atp酶活性降低51%，但Mg2+和/或DNP存在时，atp酶活性显著升高。在大脑和心脏线粒体中，atp酶活性没有变化。高温激活能使肝脏线粒体atp酶升高，而利血平处理使脑线粒体相变消除。脑线粒体总磷脂(TPL)含量增加22%。心脏线粒体TPL含量下降19%，CHL含量下降34%。观察到线粒体磷脂组成的组织特异性差异效应。利血平组肝脏线粒体膜流化程度较高。利血平治疗后，肾上腺中肾上腺素和去甲肾上腺素含量分别下降68%和77%。

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Comparative Biochemistry and Physiology Part C: Pharmacology, Toxicology and Endocrinology

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