In vitro and in vivo studies for modified ethyl cyanoacrylate regimens for sclerotherapy.

J C Lin, C W Lin, X Z Lin
{"title":"In vitro and in vivo studies for modified ethyl cyanoacrylate regimens for sclerotherapy.","authors":"J C Lin,&nbsp;C W Lin,&nbsp;X Z Lin","doi":"10.1002/1097-4636(2000)53:6<799::aid-jbm22>3.0.co;2-#","DOIUrl":null,"url":null,"abstract":"<p><p>Cyanoacrylates have known for their ability to polymerize rapidly in the presence of traces of weakly basic moieties such as water. The tissue adhesive, Histoacryl(R) (N-butyl 2-cyanoacrylate), has been reported to control bleeding through endoscopic sclerotherapy. But the commercially available Histoacryl(R) is expensive, and it has the problem like other cyanoacrylates that the glue tends to flow/run away from the point of application, which is inherent to the low viscosity, making precise application difficult. In this study, ethyl cyanoacrylate (ECA), the main constituent of \"super glue,\" was employed instead of Histoacryl(R) due to its lower cost. The aim of the research is to modify the compositions of ECA regimen and evaluate its feasibility for sclerosant application through both in vitro flow circuit model and in vivo animal tests. It was noted that the difference in the relative hardening rate between the in vitro Hepes-Tyrodes buffer flowing model and the in vivo rat model for the ECA and Histoacryl(R) was related to the existence of the blood protein, such as albumin, in the physiological milieu. It was also noticed that the ECA setting rate was greatly increased either in Hepes-Tyrodes buffer or in blood (to a comparable rate as Histoacryl(R) in vivo) by adding a few doses of caffeine, which acts as a polymerization initiator. This would lead to far better injection precision during sclerotherapy. Furthermore, in vivo histological examination for the occluded lumen of the rat's inferior vena cava and a clinical piglet portal vein occlusion experiment have suggested this new sclerosant regimen, caffeine/ECA, is of great promise in endoscopic sclerotherapy.</p>","PeriodicalId":15159,"journal":{"name":"Journal of biomedical materials research","volume":"53 6","pages":"799-805"},"PeriodicalIF":0.0000,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/1097-4636(2000)53:6<799::aid-jbm22>3.0.co;2-#","citationCount":"10","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of biomedical materials research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1002/1097-4636(2000)53:6<799::aid-jbm22>3.0.co;2-#","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 10

Abstract

Cyanoacrylates have known for their ability to polymerize rapidly in the presence of traces of weakly basic moieties such as water. The tissue adhesive, Histoacryl(R) (N-butyl 2-cyanoacrylate), has been reported to control bleeding through endoscopic sclerotherapy. But the commercially available Histoacryl(R) is expensive, and it has the problem like other cyanoacrylates that the glue tends to flow/run away from the point of application, which is inherent to the low viscosity, making precise application difficult. In this study, ethyl cyanoacrylate (ECA), the main constituent of "super glue," was employed instead of Histoacryl(R) due to its lower cost. The aim of the research is to modify the compositions of ECA regimen and evaluate its feasibility for sclerosant application through both in vitro flow circuit model and in vivo animal tests. It was noted that the difference in the relative hardening rate between the in vitro Hepes-Tyrodes buffer flowing model and the in vivo rat model for the ECA and Histoacryl(R) was related to the existence of the blood protein, such as albumin, in the physiological milieu. It was also noticed that the ECA setting rate was greatly increased either in Hepes-Tyrodes buffer or in blood (to a comparable rate as Histoacryl(R) in vivo) by adding a few doses of caffeine, which acts as a polymerization initiator. This would lead to far better injection precision during sclerotherapy. Furthermore, in vivo histological examination for the occluded lumen of the rat's inferior vena cava and a clinical piglet portal vein occlusion experiment have suggested this new sclerosant regimen, caffeine/ECA, is of great promise in endoscopic sclerotherapy.

改良氰基丙烯酸乙酯硬化治疗方案的体外和体内研究。
氰基丙烯酸酯以其在存在微量弱碱性基团(如水)时迅速聚合的能力而闻名。组织胶粘剂,组织丙烯(R) (n -丁基- 2-氰基丙烯酸酯),已报道通过内镜硬化治疗控制出血。但是市售的组织丙烯酸酯(R)价格昂贵,并且它像其他氰基丙烯酸酯一样存在胶水倾向于从应用点流动/跑开的问题,这是低粘度固有的,使得精确应用变得困难。在本研究中,由于成本较低,使用“强力胶”的主要成分氰丙烯酸乙酯(ECA)代替组织丙烯(R)。本研究的目的是通过体外血流模型和体内动物实验来改进ECA方案的组成,并评估其作为硬化剂应用的可行性。研究发现,体外Hepes-Tyrodes缓冲液流动模型与体内大鼠ECA和组织丙烯(R)相对硬化速率的差异与生理环境中血液蛋白(如白蛋白)的存在有关。我们还注意到,在Hepes-Tyrodes缓冲液或血液中,通过添加几剂量的咖啡因(作为聚合引发剂),ECA的凝固速率大大提高(与体内组织丙烯基(R)的速率相当)。这将导致在硬化治疗期间更好的注射精度。此外,对大鼠下腔静脉闭塞管腔的体内组织学检查和仔猪门静脉闭塞实验表明,咖啡因/ECA这种新的硬化方案在内镜硬化治疗中具有很大的前景。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信