Bile duct ligation and oxidative stress in the rat: effects in liver and kidney

Comparative Biochemistry and Physiology Part C: Pharmacology, Toxicology and Endocrinology Pub Date : 2000-06-01 DOI:10.1016/S0742-8413(00)00102-X

Myriam Orellana, Ramón Rodrigo, Lilian Thielemann, Viviana Guajardo

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引用次数: 109

Abstract

In the liver, seven days of bile duct ligation (BDL) decreases the cytochrome P-450 content and the UDP-glucuronyl transferase activity. Also, a decrease in the water soluble antioxidant mechanism reflected in the activities of the enzymes superoxide dismutase (SOD), catalase and the glutathione peroxidase (GTPx) was found in the liver but not in the kidney. Despite an increase in the amount of the GSH in the liver, increased lipid peroxidation is produced in the BDL rats, as indicated by the levels of malondialdehyde (MDA). The kidney responded in a different way to cholestasis, decreasing only the UDP-glucuronyl transferase activity and increasing the levels of GSH and MDA. In the red blood cells the activity of the antioxidant enzymes SOD, GTPx and catalase and the content of GSH were not modulated by cholestasis. In conclusion, disturbance of the oxidant-antioxidant balance might be responsible for cholestatic liver injury and impaired renal function in BDL rats.

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胆管结扎和氧化应激对大鼠肝脏和肾脏的影响

在肝脏中，7天胆管结扎(BDL)降低了细胞色素P-450含量和udp -葡萄糖醛酸转移酶活性。水溶性抗氧化机制的降低体现在肝脏超氧化物歧化酶(SOD)、过氧化氢酶(过氧化氢酶)和谷胱甘肽过氧化物酶(GTPx)活性的降低，而肾脏无此现象。尽管肝脏中谷胱甘肽的数量增加，但BDL大鼠的脂质过氧化也增加了，丙二醛(MDA)水平表明了这一点。肾脏以不同的方式对胆汁淤积作出反应，仅降低udp -葡萄糖醛酸转移酶活性，增加GSH和MDA水平。在红细胞中，抗氧化酶SOD、GTPx和过氧化氢酶的活性和GSH的含量不受胆汁淤积的调节。综上所述，氧化-抗氧化平衡紊乱可能是BDL大鼠胆汁淤积性肝损伤和肾功能受损的主要原因。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Comparative Biochemistry and Physiology Part C: Pharmacology, Toxicology and Endocrinology

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