Effects of gonadal steroids during pubertal development on androgen and estrogen receptor-alpha immunoreactivity in the hypothalamus and amygdala.

Journal of neurobiology Pub Date : 2000-09-05
R D Romeo, S L Diedrich, C L Sisk
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Abstract

Perinatal development is often viewed as the major window of time for organization of steroid-sensitive neural circuits by steroid hormones. Behavioral and neuroendocrine responses to steroids are dramatically different before and after puberty, suggesting that puberty is another window of time during which gonadal steroids affect neural development. In the present study, we investigated whether the presence of gonadal hormones during pubertal development affects the number of androgen receptor and estrogen receptor alpha-immunoreactive (AR-ir and ER alpha-ir, respectively) cells in limbic regions. Male Syrian hamsters were castrated either before or after pubertal development, and 4 weeks later they received a single injection of testosterone or oil vehicle 4 h prior to tissue collection. Immunocytochemistry for AR and ER alpha was performed on brain sections from testosterone-treated and oil-treated males, respectively. Adult males that had been castrated before puberty had a greater number of AR-ir cells in the medial preoptic nucleus than adult males that had been castrated after puberty. There were no significant differences in ER alpha-ir cell number in any of the brain regions examined. The demonstration that exposure to gonadal hormones during pubertal development is associated with reduced AR-ir in the medial preoptic nucleus indicates that puberty is a period of neural development during which hormones shape steroid-sensitive neural circuits.

青春期性腺激素对下丘脑和杏仁核雄激素和雌激素受体免疫反应性的影响。
围产期发育通常被认为是类固醇激素组织类固醇敏感神经回路的主要时间窗口。青春期前后对类固醇的行为和神经内分泌反应有显著差异,这表明青春期是性腺类固醇影响神经发育的另一个时间窗口。在本研究中,我们研究了青春期发育过程中性激素的存在是否会影响边缘区域雄激素受体和雌激素受体α免疫反应细胞(分别为AR-ir和ER - α -ir)的数量。雄性叙利亚仓鼠在青春期发育之前或之后被阉割,4周后在收集组织前4小时接受单次注射睾酮或油剂。分别对睾酮处理和油处理的雄性脑切片进行AR和ER α的免疫细胞化学检测。在青春期前被阉割的成年雄鼠内侧视前核的AR-ir细胞比在青春期后被阉割的成年雄鼠多。在任何被检查的大脑区域,内质网α -ir细胞数量都没有显著差异。在青春期发育期间暴露于性腺激素与内侧视前核AR-ir减少有关的证明表明,青春期是神经发育的一个时期,在此期间激素形成类固醇敏感的神经回路。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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