Urea biosensors and their application in hemodialysis--perspective of EnFET application.

Frontiers of medical and biological engineering : the international journal of the Japan Society of Medical Electronics and Biological Engineering Pub Date : 2000-01-01 DOI:10.1163/15685570052061991

W Torbicz, D G Pijanowska, M Dawgul

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引用次数: 7

Abstract

Parameters such as blood urea nitrogen concentration, normalized protein catabolic rate and Kt/V that are utilized for urea concentration measurements in blood and dialysate for the optimization of the hemodialysis process are reviewed in the paper. Basic methods of urea concentration measurement are described. Urea biosensors of the EnFET type based on the pH-sensitive Si3N4 gate FET and pNH4-sensitive FET with a Siloprene membrane containing nonactine, both of our own construction, are presented. Application of these biosensors for urea concentration measurement in blood and dialysate is described. An experimental microdialysis system with urease in detector solution and a pH-ISFET detector are described. A comparison of two dialysis procedures, with a commercial dialysate an initial of pH 5.6 and with pH kept lowered during the dialysis process applied to rats, is given.

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尿素生物传感器及其在血液透析中的应用——EnFET应用的展望。

本文综述了用于血液和透析液中尿素浓度测量的血尿素氮浓度、归一化蛋白分解代谢率和Kt/V等参数，以优化血液透析过程。介绍了尿素浓度测定的基本方法。本文提出了基于ph敏感Si3N4栅极FET和pnh4敏感pnh4膜的EnFET型尿素生物传感器，这两种传感器都是我们自己构建的。介绍了这些生物传感器在血液和透析液尿素浓度测量中的应用。介绍了一种尿素酶检测溶液和pH-ISFET检测仪的实验微透析系统。给出了两种透析方法的比较，一种是商业透析液，初始pH为5.6，另一种是在大鼠透析过程中保持较低的pH。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Frontiers of medical and biological engineering : the international journal of the Japan Society of Medical Electronics and Biological Engineering

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