Albumin facilitates zinc acquisition by endothelial cells.

D J Rowe, D J Bobilya
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引用次数: 42

Abstract

Albumin has long been observed to have a marked influence on the delivery of zinc to cells, but the mechanism of the interaction remains elusive. We examined whether albumin facilitates the acquisition of zinc by endothelial cells. Cultures of endothelial cells were used to analyze binding and acquisition of zinc and albumin to test this interaction. Our results indicate that albumin plays a role in facilitating the physiological delivery of zinc to endothelial cells. Albumin receptors that preferentially recognize albumin molecules carrying a zinc atom were demonstrated on the endothelial cell surface. Endocytosis is instrumental in albumin uptake, which was also consistently true of zinc uptake. Zinc and albumin were acquired by the cells in a 1:1 molar stoichiometry during the first 20 min of incubation in a medium with equimolar concentrations of zinc and albumin. The amount of albumin associated with the cells stabilized after 30 min, whereas the amount of zinc continued to increase. One possible explanation for this result is that a physiological route for zinc delivery into endothelial cells is by co-transport with albumin via receptor-mediated endocytosis.

白蛋白促进内皮细胞获得锌。
白蛋白长期以来一直被观察到对锌向细胞的传递有显著的影响,但这种相互作用的机制仍然是难以捉摸的。我们检查了白蛋白是否促进内皮细胞获得锌。内皮细胞培养用来分析锌和白蛋白的结合和获取,以测试这种相互作用。我们的研究结果表明,白蛋白在促进锌的生理输送到内皮细胞中起作用。在内皮细胞表面发现了优先识别携带锌原子的白蛋白分子的白蛋白受体。内吞作用有助于白蛋白的摄取,锌的摄取也同样如此。在锌和白蛋白等摩尔浓度的培养基中孵育的前20分钟,细胞以1:1的摩尔化学计量获得锌和白蛋白。与细胞相关的白蛋白的数量在30min后趋于稳定,而锌的数量继续增加。对这一结果的一种可能解释是锌通过受体介导的内吞作用与白蛋白共转运进入内皮细胞。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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