{"title":"A rapid measurement of apoptosis-associated light scatter changes using a hematology analyzer.","authors":"T Lertworasirikul, A Bunyaratvej","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>The alternative application of an automated hematology analyzer, H*3 system, has been described for the detection of apoptosis. Apoptosis induction by the topoisomerase I inhibitor, camptothecin (CAM) on several cell lines is followed by typical morphological alterations. On the H*3 cytogram, measurement of CAM-treated cells revealed an increased population of cells with reduced size suggesting cell contraction during apoptosis. The decreased LUC/Lymph ratio also indicated the enhanced degree of apoptosis directly correlated with increasing CAM concentration and/or incubation period. Quantitative analysis shows a good correlation between the H*3 measurement and flow cytometry measurements of Annexin V-fluorescein isothiocyanate-labeled method. Thus, the H*3 measurement, under an appropriate adjustment, can be used as a rapid monitor for evaluating the degree of apoptotic changes in drug susceptibility testing of homogeneous cell samples.</p>","PeriodicalId":10947,"journal":{"name":"Cytometry","volume":"42 3","pages":"215-7"},"PeriodicalIF":0.0000,"publicationDate":"2000-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Cytometry","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
The alternative application of an automated hematology analyzer, H*3 system, has been described for the detection of apoptosis. Apoptosis induction by the topoisomerase I inhibitor, camptothecin (CAM) on several cell lines is followed by typical morphological alterations. On the H*3 cytogram, measurement of CAM-treated cells revealed an increased population of cells with reduced size suggesting cell contraction during apoptosis. The decreased LUC/Lymph ratio also indicated the enhanced degree of apoptosis directly correlated with increasing CAM concentration and/or incubation period. Quantitative analysis shows a good correlation between the H*3 measurement and flow cytometry measurements of Annexin V-fluorescein isothiocyanate-labeled method. Thus, the H*3 measurement, under an appropriate adjustment, can be used as a rapid monitor for evaluating the degree of apoptotic changes in drug susceptibility testing of homogeneous cell samples.
自动血液学分析仪H*3系统的另一种应用已被描述用于检测细胞凋亡。拓扑异构酶I抑制剂喜树碱(camtothecin, CAM)对几种细胞系诱导凋亡，随后是典型的形态学改变。在H*3细胞图上，测量cam处理的细胞显示细胞数量增加，尺寸减小，表明细胞在凋亡过程中收缩。LUC/Lymph ratio的降低也表明细胞凋亡程度的增强与CAM浓度的增加和(或)孵育时间的增加直接相关。定量分析表明，Annexin v -异硫氰酸荧光素标记法的H*3测量值与流式细胞术测量值具有良好的相关性。因此，在适当调整的情况下，H*3的测定可以作为评价均质细胞样品药敏试验中细胞凋亡变化程度的快速监测指标。
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
