Spectrofluorimetric analysis of certain 4-quinolone in pharmaceuticals and biological fluids

Abstract

A highly sensitive spectrofluorimetric procedure is developed for the analysis of certain 4-quinolone antibiotics: sparfloxacin (I), oxolonic acid (II), flumequine (III) and enrofloxacin (IV) in their pharmaceutical dosage forms or in biological fluids. This procedure is based upon the intrinsic fluorescence in acetonitrile for sparfloxacin or upon the highly enhanced fluorescence obtained by the interaction of the drugs with AlCl₃. The optimum pH for the maximum fluorescence intensity is 8–8.5 for I, 5–6 for II, III and pH 3.5 for IV. The different experimental parameters that affect the fluorescence intensity were carefully studied and incorporated into the procedure.