In vitro invasiveness of human breast cancer cells is promoted by low density lipoprotein receptor-related protein.

Y Li, N Wood, P Grimsley, D Yellowlees, P K Donnelly
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引用次数: 40

Abstract

Low density lipoprotein receptor-related protein/alpha(2)-macroglobulin receptor (LRP) is a surface membrane endocytic receptor, one of whose many functions is the regulation of plasminogen activator-mediated cell migration. LRP is known to have a role in migration and invasion, but its direct involvement has been demonstrated only in non-tumour cells. We investigated six breast cancer cell lines and a normal mammary epithelial cell clone for surface and total cellular LRP expression, and confirmed that its presence corresponds to the ability to invade and migrate in vitro. We showed that LRP in the tumour cell lines is expressed at a wide range of levels: from approximately 300 to approximately 6,300 sites per cell. Four of the breast cancer cell lines expressed LRP at over 1,000 sites/cell and were markedly invasive in our assay, the remainder of the cell lines and the normal clone having far fewer LRP sites and lacking invasive ability. We further showed that the migratory and invasive abilities of a highly invasive breast cancer cell line are both inhibited by receptor-associated protein, a unique LRP ligand which normally has a solely intracellular distribution but which, when added to culture medium, can inhibit all other ligand interactions with this receptor.

低密度脂蛋白受体相关蛋白可促进人乳腺癌细胞的体外侵袭。
低密度脂蛋白受体相关蛋白/ α(2)-巨球蛋白受体(LRP)是一种表面膜内吞受体,其众多功能之一是调节纤溶酶原激活物介导的细胞迁移。已知LRP在迁移和侵袭中起作用,但其直接参与仅在非肿瘤细胞中得到证实。我们研究了6个乳腺癌细胞系和一个正常乳腺上皮细胞克隆的表面和总细胞LRP表达,并证实其存在与体外侵袭和迁移能力相对应。我们发现LRP在肿瘤细胞系中的表达水平范围很广:从每个细胞约300到约6300个位点。在我们的实验中,4个乳腺癌细胞系在1000多个位点/细胞上表达LRP,并且具有明显的侵袭性,其余细胞系和正常克隆的LRP位点要少得多,并且缺乏侵袭能力。我们进一步表明,高侵袭性乳腺癌细胞系的迁移和侵袭能力都受到受体相关蛋白的抑制,受体相关蛋白是一种独特的LRP配体,通常仅在细胞内分布,但当将其添加到培养基中时,可以抑制所有其他配体与该受体的相互作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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