Effects of a glyconutrient on macrophage functions

International journal of immunopharmacology Pub Date : 2000-04-01 Epub Date: 2000-02-21 DOI:10.1016/S0192-0561(99)00085-5

Doris L Lefkowitz , Rod Stuart , Bryan T Gnade , Erin Roberts , Stanley S Lefkowitz

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引用次数: 11

Abstract

Previous studies have shown that mannosylated bovine serum albumin (mBSA) enhances the respiratory burst (RB), phagocytosis, and killing of Candida albicans and Escherichia coli by resident murine peritoneal macrophages (Mφ). Upregulation of the above Mφ functions was associated with the binding of mBSA to the macrophage mannose receptor. The present study was done to determine if certain glyconutrients could stimulate Mφ functions in a similar manner. Resident peritoneal murine Mφ collected from C57BL/6 mice were exposed to the glyconutrients for 10 and 60 min. The RB was measured using chemiluminescence. Both phagocytosis and killing were measured after incubation with each of the following microorganisms: Candida albicans, Escherichia coli and Staphylococcus aureus. The percent phagocytosis and killing were determined using fluorescence microscopy. Results indicated that certain glyconutrients, caused a dose and time dependent effect on Mφ-induced killing of all three microorganisms.

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糖成分对巨噬细胞功能的影响

先前的研究表明，甘露糖化牛血清白蛋白(mBSA)增强了小鼠腹腔巨噬细胞(Mφ)对白色念珠菌和大肠杆菌的呼吸爆发(RB)、吞噬和杀伤作用。上述Mφ功能的上调与mBSA与巨噬细胞甘露糖受体的结合有关。目前的研究是为了确定某些糖成分是否能以类似的方式刺激Mφ功能。取C57BL/6小鼠腹膜常驻鼠Mφ，分别暴露于糖组分中10和60 min，采用化学发光法测定RB。分别与以下微生物孵育后测定吞噬和杀伤作用:白色念珠菌、大肠杆菌和金黄色葡萄球菌。荧光显微镜下测定细胞吞噬率和杀伤率。结果表明，某些糖营养成分对m φ诱导的三种微生物的杀伤均具有剂量和时间依赖性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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International journal of immunopharmacology

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