[Relationship between plasma D(-)-lactate levels and acute intestinal injury in rats following ischemia-reperfusion].

Y Yao, Z Sheng, Y Wu
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Abstract

Objective: To determine the kinetics of plasma D(-)-lactate levels in both portal and systemic circulations, and to examine whether elevated plasma D(-)-lactate would correlate to intestinal injury in rats subjected to acute intestinal ischemia-reperfusion.

Methods: Anesthetized rats underwent 75 minutes of superior mesenteric artery occlusion followed by 6 hours of reperfusion. Plasma D(-)-lactate levels were measured by an enzymatic spectrophotometric assay.

Results: It showed that intestinal ischemia for 75 minutes resulted in a significant elevation in D(-)-lactate levels in portal vein blood compared to baseline values (P < 0.05). Plasma D(-)-lactate levels had a tendency to further increase after reperfusion up to 6 hours. Similar alterations in D(-)-lactate were also found in systemic circulation, there were no significant differences between the portal and systemic circulation at any time point. Moreover, the histopathological evaluation scores were significantly correlated to the portal D(-)-lactate levels in animals at various time points (r = 0.415, P < 0.01). In addition, a remarkable rise of endotoxin concentration within portal vein was already found at the end of 75-minute ischemia (P < 0.05), reaching a peak at 2 hours post-reperfusion.

Conclusion: These data suggest that acute intestinal ischemia is associated with failure of mucosal barrier resulting in increased plasma D(-)-lactate levels in both portal and systemic blood. The subsequent reperfusion might cause further increase in D(-)-lactate levels, which correlated to the histopathological alterations. Plasma D(-)-lactate may be a useful marker of intestinal injury following both ischemia and reperfusion insults.

[血浆D(-)-乳酸水平与缺血再灌注后大鼠急性肠损伤的关系]。
目的:测定门脉循环和体循环血浆D(-)-乳酸水平的动力学,探讨血浆D(-)-乳酸水平升高是否与急性肠缺血再灌注大鼠肠道损伤有关。方法:麻醉大鼠经75分钟肠系膜上动脉阻断后再灌注6小时。用酶促分光光度法测定血浆D(-)-乳酸水平。结果:肠缺血75分钟后,门静脉血D(-)-乳酸水平较基线显著升高(P < 0.05)。再灌注6小时后,血浆D(-)-乳酸水平有进一步升高的趋势。在体循环中也发现了类似的D(-)-乳酸的变化,在任何时间点门静脉和体循环之间没有显著差异。各时间点动物门脉D(-)-乳酸水平与组织病理学评分呈显著相关(r = 0.415, P < 0.01)。门静脉内毒素浓度在缺血75 min时已明显升高(P < 0.05),再灌注后2 h达到峰值。结论:这些数据提示急性肠缺血与粘膜屏障的失效有关,导致门静脉和全身血液中血浆D(-)-乳酸水平升高。随后的再灌注可能导致D(-)-乳酸水平进一步升高,这与组织病理学改变有关。血浆D(-)-乳酸可能是肠缺血和再灌注损伤后的一个有用的标志物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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