Determination of protein deposits on RGP lenses by X-ray photoelectron spectroscopy.

The CLAO journal : official publication of the Contact Lens Association of Ophthalmologists, Inc Pub Date : 2000-01-01

H Ichijima, T Kawai, K Yamamoto, H D Cavanagh

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Abstract

Purpose: To establish a novel and objective screening method for evaluating the cleaning efficacy of contact lens care solutions for removal of protein deposits on single rigid gas permeable (RGP) lenses.

Methods: New and unworn RGP lenses containing no nitrogen atoms were incubated in a standard tear protein test solution. Protein deposition on lenses was determined by X-ray photoelectron spectroscopy (XPS) before and after standardized cleaning with commercially available care solutions (daily cleaners A, B) and a wetting/soaking solution or with 0.1w/v% sodium dodecyl sulfate (SDS) as a control. Cleaning efficacy was calculated based on the percentage of nitrogen atoms present in the total elements of the measured XPS scan spectrum.

Results: Nitrogen atoms originating from residual protein deposits on RGP lenses after cleaning were determined by XPS. The cleaning efficacy of the wetting/soaking solution was lower (74%) than that of daily cleaners A and B (95%) or SDS (96%) controls.

Conclusions: XPS determination of residual protein deposits is a novel and direct evaluation method for determining the cleaning efficacy of RGP lens care solutions for single lenses.

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用x射线光电子能谱法测定RGP透镜上的蛋白质沉积。

目的:建立一种新的、客观的筛选方法，评价隐形眼镜护理液去除单硬质透气性(RGP)镜片上蛋白质沉积物的清洁效果。方法:将不含氮原子的新晶状体和未磨损的晶状体置于标准泪液中孵育。用市售护理液(日常清洁剂A, B)和湿润/浸泡溶液或0.1w/v%十二烷基硫酸钠(SDS)作为对照，在标准化清洁前后，用x射线光电子能谱(XPS)测定镜片上的蛋白质沉积。清洁效果是根据测量的XPS扫描光谱中总元素中氮原子的百分比来计算的。结果:用XPS测定了RGP镜片清洗后残留蛋白沉积物中的氮原子。湿/浸泡液的清洁效果(74%)低于日常清洁剂A和B(95%)或SDS(96%)对照。结论:XPS法测定残留蛋白沉积是一种新的、直接的评价RGP单晶状体护理液清洁效果的方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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The CLAO journal : official publication of the Contact Lens Association of Ophthalmologists, Inc

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