GAG-augmented polysaccharide hydrogel: a novel biocompatible and biodegradable material to support chondrogenesis.

V F Sechriest, Y J Miao, C Niyibizi, A Westerhausen-Larson, H W Matthew, C H Evans, F H Fu, J K Suh
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引用次数: 301

Abstract

The quality of articular cartilage engineered using a cell-polymer construct depends, in part, on the chemical composition of the biomaterial and whether that biomaterial can support the chondrocytic phenotype. Acknowledging the supportive influence of tissue-specific matrix molecules on the chondrocytic phenotype, we have combined chondroitin sulfate-A (CSA) and chitosan, a glycosaminoglycan (GAG) analog, to develop a novel biomaterial to support chondrogenesis. Chitosan is a polycationic repeating monosaccharide of beta-1,4-linked glucosamine monomers with randomly located N-acetyl glucosamine units. Chitosan may be combined with the polyanionic CSA such that ionic crosslinking results in hydrogel formation. Bovine primary articular chondrocytes, when seeded onto a thin layer of CSA-chitosan, form discrete, focal adhesions to the material and maintain many characteristics of the differentiated chondrocytic phenotype, including round morphology, limited mitosis, collagen type II, and proteoglycan production. Our findings suggest CSA-chitosan may be well suited as a carrier material for the transplant of autologous chondrocytes or as a scaffold for the tissue engineering of cartilage-like tissue.

gag增强多糖水凝胶:一种支持软骨形成的新型生物相容性和可生物降解材料。
使用细胞聚合物构建的关节软骨工程的质量部分取决于生物材料的化学成分以及该生物材料是否能够支持软骨细胞表型。认识到组织特异性基质分子对软骨细胞表型的支持作用,我们将硫酸软骨素- a (CSA)和壳聚糖(一种糖胺聚糖(GAG)类似物)结合起来,开发了一种支持软骨形成的新型生物材料。壳聚糖是一种多阳离子重复单糖,由-1,4-氨基葡萄糖单体和随机定位的n -乙酰氨基葡萄糖单体组成。壳聚糖可与聚阴离子CSA结合,使离子交联形成水凝胶。牛原代关节软骨细胞,当播种到薄层csa -壳聚糖上时,形成离散的、局灶性的粘附,并保持分化软骨细胞表型的许多特征,包括圆形形态、有限的有丝分裂、II型胶原和蛋白多糖的产生。本研究结果提示csa -壳聚糖可作为自体软骨细胞移植的载体材料或软骨样组织工程的支架。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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