Large-scale Vero cell culture on microcarriers in a twenty-liter stirred tank fermentor.

M Y Liau, D Y Hsiun, S Y Li, C B Horng, S C Wu
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Abstract

In biotechnology, animal cell culture is an important process for the production of many biologicals such as vaccines, monoclonal antibodies, or other recombinant products. Among many established continuous cell lines, Vero cells can be maintained in many passages in cultures without inducing tumorigenicity and have been recommended by World Health Organization for the production of human biologicals. Owing to its anchorage-dependent growth characteristics, Vero cells can be grown on microcarrier in a suspension vessel where microcarrier provides the culture system with a high culture surface to volume ratio. In this paper we compared the growth kinetics of Vero cells on Cytodex 1 microcarrier in a 20-liter fermentor vs. 100 ml spinner flask culture. The kinetics of Vero cell growth in the 20-liter fermentor was similar to the results obtained from small spinner flask culture, as determined by cell specific growth rate or corresponding doubling time. The approximately 150-fold increase in culture vessel volume did not compromise the growth kinetics of Vero cells, suggesting the system is applicable for large stirred-tank fermentor cultures.

在20升搅拌罐式发酵罐中微载体上大规模Vero细胞培养。
在生物技术中,动物细胞培养是生产许多生物制品(如疫苗、单克隆抗体或其他重组产品)的重要过程。在许多已建立的连续细胞系中,Vero细胞可以在培养物中维持许多传代而不诱导致瘤性,并已被世界卫生组织推荐用于生产人类生物制品。由于其依赖于锚定的生长特性,Vero细胞可以在悬浮容器中的微载体上生长,微载体为培养系统提供了较高的培养表面积与体积比。在本文中,我们比较了Vero细胞在20升发酵罐和100毫升旋转瓶培养的Cytodex 1微载体上的生长动力学。Vero细胞在20升发酵罐中的生长动力学与小旋转瓶培养的结果相似,由细胞特定生长速率或相应的倍增时间决定。培养容器体积增加了大约150倍,但并没有影响Vero细胞的生长动力学,这表明该系统适用于大型搅拌罐发酵罐培养。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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