Large-scale Vero cell culture on microcarriers in a twenty-liter stirred tank fermentor.

Abstract

In biotechnology, animal cell culture is an important process for the production of many biologicals such as vaccines, monoclonal antibodies, or other recombinant products. Among many established continuous cell lines, Vero cells can be maintained in many passages in cultures without inducing tumorigenicity and have been recommended by World Health Organization for the production of human biologicals. Owing to its anchorage-dependent growth characteristics, Vero cells can be grown on microcarrier in a suspension vessel where microcarrier provides the culture system with a high culture surface to volume ratio. In this paper we compared the growth kinetics of Vero cells on Cytodex 1 microcarrier in a 20-liter fermentor vs. 100 ml spinner flask culture. The kinetics of Vero cell growth in the 20-liter fermentor was similar to the results obtained from small spinner flask culture, as determined by cell specific growth rate or corresponding doubling time. The approximately 150-fold increase in culture vessel volume did not compromise the growth kinetics of Vero cells, suggesting the system is applicable for large stirred-tank fermentor cultures.