Helicobacter pylori in tumor tissues of patients with advanced gastric adenocarcinoma: high prevalence but failure to detect integration.

J T Wang, C T Sung, J T Lin, T H Wang
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Abstract

Helicobacter pylori has been known to be associated with gastric adenocarcinoma by case control studies. However, significant portion of patients with gastric carcinoma are negative for H. pylori by serological test. To further detect the presence of H. pylori infection in serum and tissue of patients with gastric adenocarcinoma, paired tissues and serum samples from 32 patients with gastric adenocarcinoma were tested. Antibodies to H. pylori were tested by an enzyme linked immunosorbent assay (ELISA) and a Western blot analysis. H. pylori in tumor and non-tumor parts of gastric tissues were examined by histology and polymerase chain reaction (PCR). For serum antibody, eighteen (56%) of these patients were positive by ELISA while 24 (75%) were positive by Western blot. For tissue H. pylori genome, 14 were positive by histology while 28 (87%) were positive by PCR. Southern blot analysis of both tumor and non-tumor tissues revealed no evidence of integration of H. pylori DNA in the human genomes. These results suggest that H. pylori infection can be detected in most patients with gastric adenocarcinoma, and PCR and Western blot can further identify seronegative patients.

晚期胃腺癌患者肿瘤组织中幽门螺杆菌:高患病率但未能检测整合。
通过病例对照研究,已经知道幽门螺杆菌与胃腺癌有关。然而,相当一部分胃癌患者的血清学检测结果为幽门螺杆菌阴性。为了进一步检测胃腺癌患者血清和组织中是否存在幽门螺杆菌感染,我们对32例胃腺癌患者的配对组织和血清样本进行了检测。采用酶联免疫吸附试验(ELISA)和Western blot检测幽门螺杆菌抗体。采用组织学和聚合酶链反应(PCR)检测胃肿瘤和非肿瘤部位的幽门螺杆菌。血清抗体ELISA阳性18例(56%),Western blot阳性24例(75%)。组织幽门螺杆菌基因组组织学检测阳性14例,PCR检测阳性28例(87%)。对肿瘤和非肿瘤组织的Southern blot分析均未发现幽门螺杆菌DNA在人类基因组中整合的证据。以上结果提示,大多数胃腺癌患者均可检出幽门螺杆菌感染,PCR和Western blot可进一步鉴别血清阴性患者。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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