[Comparison of immunoassay kits for detection of staphylococcal enterotoxins produced by Staphylococcus aureus].

Abstract

Unlabelled: Four commercial kits, SET-RPLA, RIDASCREEN, SET-EIA, and TECRA, were compared for the efficiency of detecting staphylococcal enterotoxins (SE). There was no non-specific reaction for detection of SE produced by 21 Staphylococcus aureus strains from 5 outbreaks of food poisoning using SET-RPLA, SET-EIA and TECRA kits. The background of the results of RIDASCREEN kit was high and non-specific reactions were present in some strains.

In conclusion: (i) TECRA kit is suggested to be used for screening SE producing strains; (ii) SET-RPLA and RIDASCREEN kits are suitable for epidemiological investigation of SE types, but the lack of ability for detecting SEE, long time required for testing with SET-RPLA kit and high background when using RIDASCREEN kit must be overcome; and (iii) because of the complicated test procedures and the lack of ability for detecting SEE, the practicality of SET-EIA kit in screening and epidemiological research purposes is low.