[The development of shrimp blood agar for testing the hemolysis of shrimp's haemocyte by bacteria].

Abstract

A new plating medium, shrimp blood agar, was developed by using the haemolymph of shrimp plus 200 ppm rose bengal as the substrate. The colorless shrimp haemocytes were dyed by rose bengal to red. On the present agar, the hemolytic bacteria strain may show a clear zone surrounding the bacterial colony. The result on hemolysis of 45 bacteria representing 12 genera isolated from aquaculture environments against shrimp blood agar and sheep blood agar was also evaluated. There are 11 strains with different results. The study showed that, with the aim of screening the hemolytic bacteria to shrimp, shrimp blood agar might reveal a relatively quick and accurate results than that of sheep blood agar.