Molecular subtyping of the HIV-1 V3 loop sequences detected in HIV-1-positive patients in southern Taiwan.

B Shieh, C M Lee, Y M Chen, I J Su, C Li
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Abstract

Polymerase chain reaction and nucleotide sequence analysis were performed to amplify and determine the V3 loop sequences of human immunodeficiency virus type 1 (HIV-1) from ten seropositive patients at National Cheng Kung University Hospital, Tainan. The nucleotide sequences and the deduced amino acid (a. a.) sequences of these V3 regions were compared with those of known HIV-1 prototypes. The V3 loop a. a. sequences detected in eight individuals belong to subtype B which predominates in North America and Europe, whereas two individuals were infected with HIV-1 subtype E which is mainly found in the heterosexual populations of Thailand. Sequence analysis of these variant HIV-1 strains revealed a number of interesting features and a phylogenetic tree was also constructed according to the V3 loop nucleotide sequences of these variant strains and HIV-1 isolates from other parts of the world. Furthermore, our results suggest that the north vs south geographical separation in terms of HIV-1 epidemiology in Taiwan is insignificant.

台湾南部HIV-1阳性患者HIV-1 V3环序列的分子分型。
采用聚合酶链反应和核苷酸序列分析方法,对台南国立成功大学医院10例血清阳性患者的HIV-1型病毒V3环序列进行扩增和测定。将这些V3区域的核苷酸序列和推导出的氨基酸序列与已知的HIV-1原型进行比较。在8个个体中检测到的V3环a. a.序列属于在北美和欧洲占主导地位的B亚型,而2个个体感染了主要在泰国异性恋人群中发现的HIV-1 E亚型。对这些变异HIV-1毒株的序列分析揭示了许多有趣的特征,并根据这些变异毒株和世界其他地区HIV-1分离株的V3环核苷酸序列构建了系统发育树。此外,本研究结果显示台湾HIV-1流行病学的南北地理差异不显著。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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