Prognostic relevance of MIB-1 immunoreactivity, S-phase fraction, 5-bromo-2'-deoxyuridine labeling indices, and mitotic figures in gliomas.

H Struikmans, D H Rutgers, G H Jansen, H F Dullens, L Oosten, C A Tulleken, I van der Tweel, J J Battermann
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引用次数: 5

Abstract

Prognostic relevance of cell proliferation markers was evaluated in 27 glioma patients. By 1) flow cytometry (FCM), i.e., S-phase fraction (SPF), and BrdUrd-labeling index (LIfcm); 2) immunohistochemistry (IHC), i.e., BrdUrd-labeling index (LIihc) and MIB-1 immunoreactivity (MIB-1 LIihc); and 3) histologic examination, i.e., the presence or absence of cells in mitoses, were assessed. A longer local progression free survival (LPFS) was significantly associated with low SPF, low LIfcm, and low MIB-1 LIihc. For LIihc, no significant association was found. LIfcm appeared to be a more promising prognosticator than MIB-1 LIihc. In comparison with this marker, the presence or absence of mitotic figures appeared to be an even stronger prognosticator. Prognostic significance of LIfcm appeared to be of importance in low-grade gliomas. The number of patients in our study is limited. Our findings were: 1) the presence or absence of cells in mitoses (M-phase activity) appeared to be of more prognostic significance than LIfcm (S-phase activity) and MIB-1 LIihc (non-G0-phase activity); 2) of the tested experimental cell proliferation markers, LIfcm appeared to be of more prognostic significance than MIB-1 LIihc, SPF, and LIihc; and 3) LIfcm is likely to be an important prognosticator in low-grade gliomas and is, therefore, not definitive and only of potential interest.

脑胶质瘤中mb -1免疫反应性、s期分数、5-溴-2'-脱氧尿苷标记指数和有丝分裂数字与预后的相关性
在27例胶质瘤患者中评估细胞增殖标志物与预后的相关性。1)流式细胞术(FCM),即s相分数(SPF)和brdurd标记指数(LIfcm);2)免疫组织化学(IHC),即brdurd标记指数(LIihc)和MIB-1免疫反应性(MIB-1 LIihc);3)组织学检查,即有丝分裂中细胞的存在或缺失进行了评估。较长的局部无进展生存期(LPFS)与低SPF、低LIfcm和低MIB-1 LIihc显著相关。对于LIihc,没有发现显著的关联。与MIB-1 LIihc相比,LIfcm似乎是一个更有希望的预后指标。与此标志物相比,有丝分裂图的存在或缺失似乎是一个更强的预测因素。LIfcm在低级别胶质瘤中具有重要的预后意义。我们研究的患者数量有限。我们的发现是:1)有丝分裂中细胞的存在或缺失(m期活性)似乎比LIfcm (s期活性)和MIB-1 LIihc(非g0期活性)更具预后意义;2)在实验细胞增殖标志物中,LIfcm比MIB-1 LIihc、SPF和LIihc具有更大的预后意义;3) LIfcm可能是低级别胶质瘤的重要预后指标,因此不是决定性的,仅具有潜在的价值。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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