Prognostic relevance of MIB-1 immunoreactivity, S-phase fraction, 5-bromo-2'-deoxyuridine labeling indices, and mitotic figures in gliomas.

H Struikmans, D H Rutgers, G H Jansen, H F Dullens, L Oosten, C A Tulleken, I van der Tweel, J J Battermann
{"title":"Prognostic relevance of MIB-1 immunoreactivity, S-phase fraction, 5-bromo-2'-deoxyuridine labeling indices, and mitotic figures in gliomas.","authors":"H Struikmans,&nbsp;D H Rutgers,&nbsp;G H Jansen,&nbsp;H F Dullens,&nbsp;L Oosten,&nbsp;C A Tulleken,&nbsp;I van der Tweel,&nbsp;J J Battermann","doi":"10.1002/(SICI)1520-6823(1999)7:4<243::AID-ROI6>3.0.CO;2-F","DOIUrl":null,"url":null,"abstract":"<p><p>Prognostic relevance of cell proliferation markers was evaluated in 27 glioma patients. By 1) flow cytometry (FCM), i.e., S-phase fraction (SPF), and BrdUrd-labeling index (LIfcm); 2) immunohistochemistry (IHC), i.e., BrdUrd-labeling index (LIihc) and MIB-1 immunoreactivity (MIB-1 LIihc); and 3) histologic examination, i.e., the presence or absence of cells in mitoses, were assessed. A longer local progression free survival (LPFS) was significantly associated with low SPF, low LIfcm, and low MIB-1 LIihc. For LIihc, no significant association was found. LIfcm appeared to be a more promising prognosticator than MIB-1 LIihc. In comparison with this marker, the presence or absence of mitotic figures appeared to be an even stronger prognosticator. Prognostic significance of LIfcm appeared to be of importance in low-grade gliomas. The number of patients in our study is limited. Our findings were: 1) the presence or absence of cells in mitoses (M-phase activity) appeared to be of more prognostic significance than LIfcm (S-phase activity) and MIB-1 LIihc (non-G0-phase activity); 2) of the tested experimental cell proliferation markers, LIfcm appeared to be of more prognostic significance than MIB-1 LIihc, SPF, and LIihc; and 3) LIfcm is likely to be an important prognosticator in low-grade gliomas and is, therefore, not definitive and only of potential interest.</p>","PeriodicalId":20894,"journal":{"name":"Radiation oncology investigations","volume":"7 4","pages":"243-8"},"PeriodicalIF":0.0000,"publicationDate":"1999-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/(SICI)1520-6823(1999)7:4<243::AID-ROI6>3.0.CO;2-F","citationCount":"5","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Radiation oncology investigations","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1002/(SICI)1520-6823(1999)7:4<243::AID-ROI6>3.0.CO;2-F","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 5

Abstract

Prognostic relevance of cell proliferation markers was evaluated in 27 glioma patients. By 1) flow cytometry (FCM), i.e., S-phase fraction (SPF), and BrdUrd-labeling index (LIfcm); 2) immunohistochemistry (IHC), i.e., BrdUrd-labeling index (LIihc) and MIB-1 immunoreactivity (MIB-1 LIihc); and 3) histologic examination, i.e., the presence or absence of cells in mitoses, were assessed. A longer local progression free survival (LPFS) was significantly associated with low SPF, low LIfcm, and low MIB-1 LIihc. For LIihc, no significant association was found. LIfcm appeared to be a more promising prognosticator than MIB-1 LIihc. In comparison with this marker, the presence or absence of mitotic figures appeared to be an even stronger prognosticator. Prognostic significance of LIfcm appeared to be of importance in low-grade gliomas. The number of patients in our study is limited. Our findings were: 1) the presence or absence of cells in mitoses (M-phase activity) appeared to be of more prognostic significance than LIfcm (S-phase activity) and MIB-1 LIihc (non-G0-phase activity); 2) of the tested experimental cell proliferation markers, LIfcm appeared to be of more prognostic significance than MIB-1 LIihc, SPF, and LIihc; and 3) LIfcm is likely to be an important prognosticator in low-grade gliomas and is, therefore, not definitive and only of potential interest.

脑胶质瘤中mb -1免疫反应性、s期分数、5-溴-2'-脱氧尿苷标记指数和有丝分裂数字与预后的相关性
在27例胶质瘤患者中评估细胞增殖标志物与预后的相关性。1)流式细胞术(FCM),即s相分数(SPF)和brdurd标记指数(LIfcm);2)免疫组织化学(IHC),即brdurd标记指数(LIihc)和MIB-1免疫反应性(MIB-1 LIihc);3)组织学检查,即有丝分裂中细胞的存在或缺失进行了评估。较长的局部无进展生存期(LPFS)与低SPF、低LIfcm和低MIB-1 LIihc显著相关。对于LIihc,没有发现显著的关联。与MIB-1 LIihc相比,LIfcm似乎是一个更有希望的预后指标。与此标志物相比,有丝分裂图的存在或缺失似乎是一个更强的预测因素。LIfcm在低级别胶质瘤中具有重要的预后意义。我们研究的患者数量有限。我们的发现是:1)有丝分裂中细胞的存在或缺失(m期活性)似乎比LIfcm (s期活性)和MIB-1 LIihc(非g0期活性)更具预后意义;2)在实验细胞增殖标志物中,LIfcm比MIB-1 LIihc、SPF和LIihc具有更大的预后意义;3) LIfcm可能是低级别胶质瘤的重要预后指标,因此不是决定性的,仅具有潜在的价值。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信