Infrared spectroscopy of human tissue. V. Infrared spectroscopic studies of myeloid leukemia (ML-1) cells at different phases of the cell cycle

Biospectroscopy Pub Date : 1999-07-21 DOI:10.1002/(SICI)1520-6343(1999)5:4<219::AID-BSPY2>3.0.CO;2-O

Susie Boydston-White, Tamara Gopen, Sandra Houser, Jill Bargonetti, Max Diem

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引用次数: 173

Abstract

Infrared spectra of myeloid leukemia (ML-1) cells are reported for cells derived from an asynchronous, exponentially growing culture, as well as for cells that were fractionated according to their stage within the cell division cycle. The observed results suggest that the cells' DNA is detectable by infrared spectroscopy mainly when the cell is in the S phase, during the replication of DNA. In the G1 and G2 phases, the DNA is so tightly packed in the nucleus that it appears opaque to infrared radiation. Consequently, the nucleic acid spectral contributions in the G1 and G2 phases would be mostly that of cytoplasmic RNA. These results suggest that infrared spectral changes observed earlier between normal and abnormal cells may have been due to different distributions of cells within the stages of the cell division cycle. © 1999 John Wiley & Sons, Inc. Biospectroscopy 5: 219–227, 1999

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人体组织的红外光谱。5 .细胞周期不同阶段髓性白血病(ML-1)细胞的红外光谱研究

据报道，髓系白血病(ML-1)细胞的红外光谱来自于异步、指数生长培养的细胞，以及根据细胞分裂周期内的阶段进行分离的细胞。观察结果表明，红外光谱主要在细胞处于S期DNA复制时检测到细胞的DNA。在G1期和G2期，DNA被紧紧地包裹在细胞核中，以至于红外线辐射对它来说是不透明的。因此，在G1期和G2期的核酸光谱贡献将主要是细胞质RNA。这些结果表明，早期在正常和异常细胞之间观察到的红外光谱变化可能是由于细胞在细胞分裂周期阶段的不同分布。©1999 John Wiley &儿子,Inc。生物光谱学学报，2009,31 (2):557 - 557

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Biospectroscopy

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