[Effects of blocking CD18-mediated leukocyte adhesion on the survival of the island flap].

Zhonghua zheng xing shao shang wai ke za zhi = Zhonghua zheng xing shao shang waikf [i.e. waike] zazhi = Chinese journal of plastic surgery and burns Pub Date : 1998-01-01

H Gu, Z Lin, Q Liu

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Abstract

Objective: This study was to investigate the role of leukocyte and leukocyte adhesion in tissue injury from ischemia and reperfusion.

Methods: The experiment utilized the monoclonal antibody (mAb) directed to the leukocyte adhesion glycoprotein CD18 to block leukocyte adhesion and aggregation in an island flap model in rats. Tissue content of myeloperoxidase (MPO) and malondialdehyde (MDA) were detected after transient treatment with either saline or mAb directed to CD18. Flap survival was assessed 7 days afterwards.

Results: The content of both MPO and MDA was significantly increased with 8 h ischemia and 1 h reperfusion of the flap. The treatment with anti-CD18 mAb significantly decreased the levels of MPO and MDA and also significantly improved the survival of flaps compared with the saline-treated controls.

Conclusion: CD18-mediated leukocyte adhesion plays an important role in tissue injury from ischemia and reperfusion. Blocking leukocyte adhesion can attenuate leukocyte-mediated injury, providing protective effects on island flaps.

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[阻断cd18介导的白细胞粘附对岛状皮瓣存活的影响]。

目的:探讨白细胞及其粘附在缺血再灌注损伤中的作用。方法:利用白细胞粘附糖蛋白CD18单克隆抗体(mAb)阻断大鼠岛状皮瓣模型中白细胞的粘附和聚集。用生理盐水或针对CD18的单抗短暂处理后，检测组织中髓过氧化物酶(MPO)和丙二醛(MDA)的含量。7天后评估皮瓣存活。结果:皮瓣缺血8 h、再灌注1 h时，MPO和MDA含量均显著升高。与盐水处理的对照组相比，抗cd18单抗治疗显著降低了MPO和MDA的水平，也显著提高了皮瓣的存活率。结论:cd18介导的白细胞粘附在缺血再灌注损伤中起重要作用。阻断白细胞粘附可减轻白细胞介导的损伤，对岛状皮瓣提供保护作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Zhonghua zheng xing shao shang wai ke za zhi = Zhonghua zheng xing shao shang waikf [i.e. waike] zazhi = Chinese journal of plastic surgery and burns

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