Gene regulation of atrial natriuretic peptide A, B, and C receptors in rat glomeruli.

Experimental nephrology Pub Date : 1999-07-01 DOI:10.1159/000020621

K Itoh, H Nonoguchi, N Shiraishi, K Tomita

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引用次数: 12

Abstract

Background and methods: Atrial natriuretic peptide (ANP) has three types of receptor. We investigated the gene regulation of three types of ANP receptors (ANPR-A, B, and C) in rat glomeruli using reverse transcription coupled with competitive polymerase chain reaction (PCR).

Results: Competitive PCR revealed that ANPR-C mRNA expression was most abundant (ANPR-C > A >> B) in glomeruli from control rats among mRNA expressions of three receptors, which were 20- to 15,000-fold higher than those in inner medullary collecting ducts. Two days' dehydration caused reversible decreases of ANPR-A, B, and C mRNAs by 50-80%. To determine the mechanisms of down-regulation of mRNA expression, isolated glomeruli were incubated in isotonic or hypertonic solution. Hyperosmolality induced by NaCl, mannitol or raffinose caused significant increases of ANPR-A, B, and C mRNA expression. Hypertonicity by urea showed smaller effects. ANP stimulated the expression of ANPR-A, B, and C mRNA in vitro.

Conclusion: These results indicate that dehydration caused reversible decreases of ANPR-A, B, and C mRNA expression in glomeruli, and these decreases were not caused by increased plasma osmolality but probably by lower circulating levels of ANP.

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大鼠肾小球心房利钠肽A、B和C受体的基因调控。

背景与方法:心房利钠肽(ANP)有三种受体。我们利用反转录和竞争性聚合酶链反应(PCR)技术研究了大鼠肾小球中三种ANP受体(ANPR-A、B和C)的基因调控。结果:竞争性PCR结果显示，三种受体mRNA表达量中，对照大鼠肾小球中ANPR-C mRNA表达量最高(ANPR-C > A > B)，比髓内集管高20 ~ 1.5万倍。脱水2天导致ANPR-A、B和C mrna可逆降低50-80%。为了确定mRNA表达下调的机制，将离体肾小球置于等渗或高渗溶液中孵育。NaCl、甘露醇和棉子糖诱导的高渗可显著增加ANPR-A、B和C mRNA的表达。尿素的高渗作用较小。ANP刺激体外ANPR-A、B和C mRNA的表达。结论:脱水引起肾小球内ANPR-A、B和C mRNA表达的可逆性降低，这种降低可能与血浆渗透压升高无关，而可能与循环ANP水平降低有关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Experimental nephrology

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