Multiple-pulse-mediated electrofusion of intact erythrocyte onto human term placental amnion

Abstract

The creation of surface modified human term placental amnion by electrofusing human cells onto its surface has been thought of. A multiple-pulse electrofusion protocol with 10 square pulses of 10-μs pulse length, and electric field of 0.2 kV cm⁻¹, can make erythrocyte–amnion tissue electrofusion possible. The protocol devised merge the cell-tissue-adherence steps with fusogenic pulse. The finding opens up a new avenue of cell electrofusion onto human tissue with minimal procedural complexities.