Examination of the relationship between parameters to determine electropermeability of Saccharomyces cerevisiae

Masafumi Muraji , Hiroaki Taniguchi , Wataru Tatebe , Hermann Berg
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引用次数: 15

Abstract

A rectangular electric pulse was applied to Saccharomyces cerevisiae suspensions in NaCl solutions. The relationship between field strength, pulse width and conductivity of extracellular media of key—factors to determine the yield of electropermeability—was examined at the time when the same permeability occurred. The results showed that the dependence of the yield of permeability upon the width of applied pulse was mutually related with the conductivity of extracellular media. Namely at one field strength, the value of pulse width is inversely proportional to that of conductivity of media and its relationship holds true for any field strength. Further, the relationship between parameters considered bears a close resemblance to that recognized between stress amplitude and the number of cycles to failure in the fatigue fracture of materials.

测定酿酒酵母电渗透性参数关系的研究
在NaCl溶液中对酿酒酵母悬浮液施加矩形电脉冲。在相同渗透率发生时,考察了电场强度、脉冲宽度和细胞外介质电导率(决定电渗透率产率的关键因素)之间的关系。结果表明,渗透率随脉冲宽度的变化与细胞外介质的电导率密切相关。即在一个场强下,脉冲宽度的值与介质电导率的值成反比,其关系对任何场强都成立。此外,所考虑的参数之间的关系与材料疲劳断裂中应力幅值与失效循环次数之间的关系非常相似。
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