Heterogeneity of in vitro radiosensitivity in human bladder cancer cells.

A R Barnetson, D Banasiak, R J Fisher, H Mameghan, J C Ribeiro, K Brown, J L Brown, S M O'Mara, P J Russell
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引用次数: 13

Abstract

Human bladder cancer is often heterogeneous containing biologically different populations. Radiotherapy plus chemotherapy is the most common treatment for invasive disease. However few studies have investigated the role of heterogeneity in determining radiosensitivity. The radiation sensitivities of a parent human bladder cancer cell line (UCRU-BL-17CL) and nine cloned cell lines derived from it were determined. These cloned cell lines were previously shown to exhibit different biological characteristics when grown in nude mice. Radiation sensitivity was determined using both MTT and clonogenic assays. The radiobiological parameters, alpha,beta, and surviving fractions at 2 Gy and 8 Gy from the linear-quadratic model, were used to assess radiation sensitivity in the statistical analyses. The nine clones differed in radiosensitivity by both assays. By MTT, but not by the clonogenic assay, their radiation sensitivities were relatively consistent within each of the three biological groups (non-tumorigenic, tumorigenic, invasive); invasive clones were more sensitive than those of the non-tumorigenic and the tumorigenic groups for all the three-test criteria. The heterogeneity exhibited by this cell line may explain some of the variations in the clinical responses seen in the radiation treatment of invasive bladder cancer.

人膀胱癌细胞体外放射敏感性的异质性。
人类膀胱癌通常是异质的,包含生物学上不同的人群。放疗加化疗是侵袭性疾病最常用的治疗方法。然而,很少有研究调查异质性在确定放射敏感性中的作用。测定了一株人膀胱癌亲本细胞株(UCRU-BL-17CL)及其衍生的9株克隆细胞株的辐射敏感性。这些克隆细胞系在裸鼠体内生长时显示出不同的生物学特性。用MTT和克隆测定法测定辐射敏感性。在统计分析中,使用线性二次模型中的放射生物学参数α、β和存活分数(2gy和8gy)来评估辐射敏感性。这9个克隆在两种检测中放射敏感性不同。通过MTT,而不是克隆性试验,他们的辐射敏感性在三个生物组(非致瘤性、致瘤性、侵袭性)中相对一致;侵袭性克隆对所有三项检测标准的敏感性均高于非致瘤性组和致瘤性组。这种细胞系的异质性可以解释侵袭性膀胱癌放射治疗中临床反应的一些差异。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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