[Tylosin-specific proliferation in vitro of the peripheral blood mononuclear cells in occupational tylosin allergy].

Problemi na khigienata Pub Date : 1997-01-01
D Baltadzhieva, S Bocheva, I Altŭnkova, M Demireva
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Abstract

Tylosin-specific lymphocyte proliferation in vitro and its restriction of HLA class II antigens have been investigated in 21 allergic workers exposed to tylosin. Nine workers from tylosin industry without allergic symptoms and five nonexposed nonallergic subjects served as controls. The results showed a significantly higher lymphocyte proliferative response to tylosin in allergic workers compared to control groups. Lymphocyte proliferation was observed in five workers with allergic complaints but negative skin tests. The tylosin concentration leading to maximal proliferative response varied from 10 to 1000 micrograms/ml among individuals. Six from seven workers with maximal response to the lowest concentration of tylosin carried HLA-DQ2 antigen. Lymphocyte from most control subjects did not respond in vitro to tylosin. Weak proliferative response to tylosin was observed in two workers without clinical symptoms of allergy. No association was found between lymphocyte reactivity to PHA and tylosin in the three studied groups. In conclusion, tylosin leads to a specific activation of T lymphocytes in occupationally sensitized workers. Possibly, the T cell recognition of the hapten/protein complex is restricted by HLA-DQ2 antigen in tylosin occupational allergy. Lymphocyte stimulation test can be used for the diagnosis of tylosin occupational allergy, as well as for the detection of latently sensitized workers.

[职业性泰乐素过敏患者外周血单个核细胞泰乐素特异性体外增殖]。
本文研究了21例泰乐素过敏工人泰乐素特异性淋巴细胞体外增殖及其HLAⅱ类抗原的限制。9名泰乐菌素行业无过敏症状的工人和5名未暴露的非过敏受试者作为对照。结果显示,与对照组相比,过敏工人对泰络素的淋巴细胞增殖反应明显更高。在5名有过敏症状但皮肤试验阴性的工人中观察到淋巴细胞增殖。导致最大增殖反应的泰络素浓度在个体之间从10到1000微克/毫升不等。7名工人中有6名对最低浓度的泰洛菌素反应最大,携带HLA-DQ2抗原。大多数对照对象的淋巴细胞对泰洛菌素没有反应。两名无临床过敏症状的工人对泰乐素的增殖反应较弱。在三个研究组中,淋巴细胞对PHA的反应性和泰络素之间没有发现关联。综上所述,泰络素导致职业致敏工人的T淋巴细胞特异性活化。可能在泰络素职业性过敏中,T细胞对半抗原/蛋白复合物的识别受到HLA-DQ2抗原的限制。淋巴细胞刺激试验可用于泰络素职业过敏的诊断,也可用于发现潜伏致敏的工人。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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