E Bertino, A Coscia, S Costa, D Farinasso, G Prandi, C Fabris, M Cavaletto, M G Giuffrida, A Conti
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引用次数: 0
Abstract
Objectives: To analyze the presence of bovine beta-LG in breast milk.
Methods: Human milk samples from 14 healthy non-atopic women on diets with different cow's milk contents were examined. The total concentration of beta-LG immuno-like proteins (beta-LGIP) was determined by enzyme linked immunosorbent assay (ELISA). Identification of antigens was done by N-terminal sequencing.
Results: beta-LGIP reactivity of the milk from subjects on different diets was not significantly different. Human lactoferrin, beta-casein and alpha-lactalbumin, were identified as cross-reacting antigens.
Conclusions: False-positive results in ELISA determinations of bovine beta-LG in human milk might be due to cross-reactions between polyclonal antibodies and different protein antigens.
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