[Effect of tocopherol deficiency and additional administration of tocopherol, dibunol and disodium selenite on enzyme activity in rat liver, which take part in the biotransformation of nitrosodimethylamine].

Ukrainskii biokhimicheskii zhurnal (1978) Pub Date : 1998-07-01

V M Isotshyn, O O Pentiuk, M V Matviĭchuk

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Abstract

The alimentary tocopherol deficiency is accompanied by decreased hydroxylase, demethylase, NADH- and NADPH-reductase, aldehyde dehydrogenase, arylesterase and glutathione reductase activity in rat's liver. It decreased the reduced glutathione and increased it's oxidized form concentration in the tocopherol deficient animals. The stability of microsomal membrane is decreased to solubilizing action of deoxycholate and trypsin. This changes, possibly, caused elevation of alteration of function enzyme's and microsomal membrane after nitrosodimethylamine (NDMA) administration in deficient rats. The 7-days injection of tocopherol (20 and 100 mg/kg), dibunol (80 mg/kg), sodium selenite (30 mkg/kg) increased aldehyde dehydrogenase, esterase, glutathione-dependent enzymes activity and increased of reduced glutathione concentration in liver, suppressed lipid peroxidation and increased survival rats after lethal dose carcinogen treatment. Supplementation of tocopherol decreased harmful action of nitrosodimethylamine on microsomal membrane and enzymes activity.

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[生育酚缺乏和额外给予生育酚、二酚和亚硒酸二钠对参与亚硝基二甲胺生物转化的大鼠肝脏酶活性的影响]。

消化道生育酚缺乏可导致大鼠肝脏羟化酶、去甲基化酶、NADH-和nadph -还原酶、醛脱氢酶、芳基酯酶和谷胱甘肽还原酶活性降低。在生育酚缺乏的动物体内降低还原型谷胱甘肽并增加其氧化形式的浓度。脱氧胆酸盐和胰蛋白酶的溶解作用降低了微粒体膜的稳定性。这种变化可能引起亚硝基二甲胺(NDMA)给药后功能酶和微粒体膜的改变升高。致死剂量致癌物治疗后7 d内注射生育酚(20、100 mg/kg)、二酚(80 mg/kg)、亚硒酸钠(30 mg/kg)，可提高肝脏醛脱氢酶、酯酶和谷胱甘肽依赖酶活性，增加还原性谷胱甘肽浓度，抑制脂质过氧化，提高存活率。补充生育酚可降低亚硝基二甲胺对微粒体膜的有害作用和酶活性。

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Ukrainskii biokhimicheskii zhurnal (1978)

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