Equine cardiodilatin/ atrial natriuretic peptide. Primary structure and immunohistochemical localization in auricular cardiocytes.

Acta anatomica Pub Date : 1998-01-01
R Richter, H Mägert, H Mifune, P Schulz-Knappe, W Forssmann
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Abstract

Cardiodilatin (CDD)/atrial natriuretic peptide (ANP) is a 28-amino acid peptide hormone known to be synthesized in the heart of a large number of different vertebrates. It plays an important role in the regulation of blood pressure and natriuresis/diuresis. Since the cardiovascular system of the horse has to meet the highest requirements concerning its physiological performance, we intended to characterize the cardiodilatin/atrial natriuretic peptide system of this species. By means of immunohistochemistry and immunoelectron microscopy, we precisely identified auricular cardiocytes as the loci of CDD/ANP synthesis. Using aortic smooth muscle relaxation assay and CDD/ANP-ELISA, we succeeded in isolating the biologically active prohormone. We subsequently cloned the equine cDNA of the CDD/ANP precursor protein and deduced its primary sequence. The entire precursor protein is in good agreement with the CDD/ANP prohormones of other mammals. The deduced theoretical average Mr of equine CDD/ANP-1-126 is 13,764, corresponding to the molecular weight of purified peptide determined by ESI-MS. Our findings suggest that equine CDD/ANP is produced in auricular cardiocytes and the predominant storage form of CDD/ANP in the auricle is the prohormone CDD/ANP-1-126.

马心舒张素/心房利钠肽。耳廓心肌细胞的原代结构和免疫组织化学定位。
心舒张素(CDD)/心房利钠肽(ANP)是一种由28个氨基酸组成的肽激素,已知在许多不同脊椎动物的心脏中合成。它在调节血压和利钠/利尿中起重要作用。由于马的心血管系统必须满足其生理性能的最高要求,我们打算表征该物种的心舒张素/心房利钠肽系统。通过免疫组织化学和免疫电镜技术,我们精确地鉴定出耳廓心肌细胞是CDD/ANP合成的位点。采用主动脉平滑肌舒张实验和CDD/ANP-ELISA,成功分离出具有生物活性的原激素。随后,我们克隆了马CDD/ANP前体蛋白cDNA,并推导出其一级序列。整个前体蛋白与其他哺乳动物的CDD/ANP原激素一致。推导出马CDD/ANP-1-126的理论平均Mr为13764,与ESI-MS测定的纯化肽分子量相对应。我们的研究结果表明,马CDD/ANP是在耳廓心肌细胞中产生的,CDD/ANP在耳廓中的主要储存形式是激素原CDD/ANP-1-126。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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