Changes in choroidal innervation in Royal College of Surgeons rats with hereditary retinal degeneration.

Acta anatomica Pub Date : 1998-01-01 DOI:10.1159/000046475
M Rexer, C A May, E Lütjen-Drecoll
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引用次数: 5

Abstract

In Royal College of Surgeons (RCS) rats with hereditary retinal degeneration loss of retinal pigmented epithelium (RPE) and choriocapillaris is most pronounced in the upper-temporal quadrant. To investigate whether changes in choroidal vasodilative innervation might be involved in the RPE degeneration, we analyzed whole mount preparations of the retina and choroid stained for nitric oxide synthase and for NADPH-diaphorase (d) of 19 dystrophic RCS rats and 24 age-matched congenic controls of different age groups. Density of NADPH-d-positive nerve fibers was quantitatively evaluated in the upper-temporal and lower-nasal quadrant. Our results revealed that even in control animals there were much less positively stained nerve fibers in the upper-temporal than in the lower-nasal quadrant. Nerve fiber density in both quadrants increased for up to 3 months and remained nearly constant throughout life. In the dystrophic animals up to 3 months of age nerve fiber density was similar to that seen in the controls. In dystrophic animals older than 3 months nerve fiber density in the upper-temporal quadrant decreased significantly, whereas density in the lower-nasal quadrant revealed nearly the same values as in the age-matched controls. Decrease of NADPH-d stained nerve fibers in this quadrant occurred prior to the vascular changes in the choriocapillaris. In the retina of RCS dystrophic rats an increase of NADPH-d-positive amacrine cells was found only in 3-month-old animals. Most of these cells were located in the vicinity of irregularly arranged branches of the central retinal artery. In animals 5 months of age and older the number of cells decreased to the same values found in controls, so that we assume that increase of NADPH-d-positive amacrine cells is involved in capillary degeneration or sprouting.

遗传性视网膜变性大鼠脉络膜神经支配的变化。
在英国皇家外科医学院(RCS)的研究中,遗传性视网膜变性大鼠视网膜色素上皮(RPE)和绒毛膜毛细血管的损失在颞上象限最为明显。为了探讨脉络膜血管舒张神经的改变是否与RPE变性有关,我们分析了19只营养不良的RCS大鼠和24只不同年龄组的年龄匹配的基因对照组的视网膜和脉络膜的一氧化氮合酶和nadph -脱氢酶(d)染色。定量评价上颞和下鼻象限nadph -d阳性神经纤维的密度。我们的结果显示,即使在对照动物中,上颞叶的阳性染色神经纤维也比下鼻象限的少得多。两个象限的神经纤维密度在长达3个月的时间里都有所增加,并且在整个生命中几乎保持不变。在3个月大的营养不良动物中,神经纤维密度与对照组相似。在3个月以上的营养不良动物中,上颞象限的神经纤维密度显著下降,而下鼻象限的密度与年龄匹配的对照组几乎相同。这一象限NADPH-d染色的神经纤维减少发生在绒毛膜毛细血管改变之前。在RCS营养不良大鼠的视网膜中,仅在3月龄的动物中发现nadph -d阳性无分泌细胞的增加。这些细胞大多位于视网膜中央动脉不规则排列的分支附近。在5个月或更大的动物中,细胞数量减少到与对照组相同的值,因此我们假设nadph -d阳性无毛细胞的增加与毛细血管变性或发芽有关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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