Immunocytochemistry of fibronectin and endothelin-1 in the cavernous body of postnatal rabbit penises.

Acta anatomica Pub Date : 1998-01-01 DOI:10.1159/000046465
T Nomiyama, Y Doi, H Kudo, H Furukawa, K Hamasaki, S Fujimoto
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引用次数: 3

Abstract

The differentiating cavernous body (CB) of postnatal rabbit penises was examined with a special reference to immunolocalizations for fibronectin (FN) and endothelin-1 (ET-1). At postnatal day 1, the CBs were embedded by an abundance of mesenchymal cells (MCs), and some of them were closely associated with endothelial cells of preexisting capillaries. Our electron micrographs indicated that such MCs are successively incorporated into the capillary endothelium as vasoformative cells. At this period, vascular sprouts of the helicine artery (HA), which were associated with the MCs, arose from the deep penile artery, and the transformation of such cells to endothelial and medial muscle ones was also indicated, and some MCs appeared to differentiate to epithelioid cells in the media. Immunoreactions for FN were preferentially localized in the rough endoplasmic reticulum (rER) and along the plasma membrane of such vasoformative MCs, and on the extracellular matrix components which connect these MCs with sprouts of both growing capillaries and HA. These findings suggest that FN, which is produced in the rER of the MCs, plays a crucial role in the mechanical linkage during the incorporation of vasoformative MCs into these penile vessels. Immunoreactions for ET-1 were preferentially localized on Weibel-Palade bodies in endothelial cells of the HA, implying the involvement of this peptide in the regulation of the local blood flow in this vessel.

兔出生后阴茎海绵体纤维连接蛋白和内皮素-1的免疫细胞化学。
本文对兔出生后阴茎的分化海绵体(CB)进行了研究,特别关注了纤维连接蛋白(FN)和内皮素-1 (ET-1)的免疫定位。出生后第1天,CBs被大量间充质细胞(MCs)包埋,其中一些细胞与预先存在的毛细血管内皮细胞密切相关。电镜图显示,这些MCs作为血管形成细胞陆续进入毛细血管内皮。这一时期,与MCs相关的螺旋动脉(helicine artery, HA)的血管芽从阴茎深动脉产生,这些细胞也向内皮细胞和内侧肌肉细胞转化,一些MCs在培养基中分化为上皮样细胞。FN的免疫反应优先定位于粗内质网(rER)和这些血管形成型MCs的质膜,以及连接这些MCs与生长中的毛细血管芽和HA的细胞外基质成分。这些发现表明,在血管形成性MCs进入这些阴茎血管的过程中,由MCs的内质网产生的FN在机械连接中起着至关重要的作用。ET-1的免疫反应优先定位于HA内皮细胞的Weibel-Palade小体,这意味着该肽参与了血管局部血流的调节。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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